Description

3,3-Difluoro-3H-Diazirine is a highly reactive, small-molecule carbene precursor widely used in photoaffinity labeling and cross-linking applications. Its core value lies in its ability to generate a reactive difluorocarbene species upon photolysis, enabling the covalent tagging of biomolecules for structural and interaction studies. This compound is essential for researchers and manufacturers in the pharmaceutical, biotechnology, and chemical synthesis sectors who require precise tools for probing protein-ligand interactions, mapping binding sites, and developing novel diagnostic agents.

Application

• Photoaffinity Labeling (PAL): A critical tool for identifying and characterizing drug targets, protein receptors, and enzyme active sites by forming irreversible covalent bonds upon UV light activation.
• Bioconjugation and Cross-linking: Used to create stable cross-links between biomolecules (e.g., protein-protein, protein-nucleic acid) for structural biology studies and assay development.
• Proteomics and Chemical Biology: Employed in activity-based protein profiling (ABPP) to discover and validate novel therapeutic targets and understand metabolic pathways.
• Materials Science Surface Modification: Facilitates the covalent attachment of functional groups to polymers, nanoparticles, and other surfaces to alter physicochemical properties.
• Organic Synthesis Intermediate: Serves as a versatile reagent for introducing difluoromethylene (CF2) groups into complex organic molecules, valuable in medicinal chemistry for modulating compound properties.
• Development of Diagnostic Kits: Integral in creating labeled probes and tracers for immunoassays, imaging agents, and other diagnostic applications.

Basic Information

Product Name	3,3-Difluoro-3H-Diazirine
CAS No.	693-85-6
Molecular Formula	C2H2F2N2
Molecular Weight	92.05 g/mol
Synonyms	Difluorodiazirine; 3,3-Difluorodiaziridine; Difluoromethylene Diaziridine; 3H-Diazirine, 3,3-difluoro-; Diaziridine, difluoromethylene-; Carbene, difluoro-, precursor; Photoaffinity labeling reagent
EINECS	Contact for details

Quality Control

Our 3,3-Difluoro-3H-Diazirine is produced and handled under strict quality protocols to ensure batch-to-batch consistency and reliability for sensitive research applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity assessment and NMR for structural confirmation. A detailed Certificate of Analysis (COA) documenting identity, purity, and stability is provided with every shipment to support your quality assurance and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C (refrigerated) or as specified on the label or COA. Due to its highly volatile nature, allow the container to equilibrate to room temperature before opening to minimize pressure buildup. Keep away from heat, sparks, and open flame. Store in a cool, dry, well-ventilated place, separate from incompatible materials.

Specification

Item	Specification
Appearance	Colorless to pale yellow liquid
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.