Description

L-Leucine-D3-n-Fmoc (Methyl-D3) is a deuterium-labeled, N-Fmoc protected derivative of the essential amino acid L-leucine, specifically designed for advanced research and development. This high-purity, isotopically labeled compound is critical for applications requiring precise molecular tracking and analysis, such as in pharmacokinetic and metabolic studies. It is an indispensable tool for pharmaceutical researchers, analytical chemists, and biochemists working in drug discovery, proteomics, and mass spectrometry.

Application

• Internal Standard for Mass Spectrometry (MS): Used as a quantitative internal standard in LC-MS and GC-MS analyses to ensure accuracy in measuring unlabeled L-leucine or related peptides in complex biological matrices.
• Metabolic Pathway Tracing: Employed in stable isotope labeling (SIL) experiments to study amino acid metabolism, protein turnover rates, and biosynthetic pathways in cell cultures and animal models.
• Pharmaceutical Research & Development: Serves as a key building block in the synthesis of deuterated drug candidates for improved metabolic stability and for conducting Absorption, Distribution, Metabolism, and Excretion (ADME) studies.
• Peptide Synthesis: Acts as a protected, labeled amino acid precursor in solid-phase and solution-phase peptide synthesis for creating isotopically labeled peptides used as probes or standards.
• Proteomics and Biomarker Discovery: Facilitates relative and absolute quantification in quantitative proteomics workflows, such as in SILAC (Stable Isotope Labeling by Amino acids in Cell culture) or as a spike-in standard.
• NMR Spectroscopy: Useful as a probe in Nuclear Magnetic Resonance (NMR) spectroscopy studies, where the deuterium labels can simplify spectra and provide structural insights.

Basic Information

Product Name	L-Leucine-D3-n-Fmoc (Methyl-D3)
CAS No.	538372-74-6
Molecular Formula	C₂₁H₂₀D₃NO₄
Molecular Weight	356.44 g/mol
Synonyms	Fmoc-Leu(D3)-OH; (2S)-2-[(9H-Fluoren-9-ylmethoxy)carbonyl]amino-4-methyl-d3-pentanoic acid; N-[(9H-Fluoren-9-ylmethoxy)carbonyl]-L-leucine-d3; Fmoc-L-leucine-d3; Fmoc-Leu(D3); L-Leucine-d3, N-[(9H-fluoren-9-ylmethoxy)carbonyl]-; (S)-2-((((9H-Fluoren-9-yl)methoxy)carbonyl)amino)-4-methyl-d3-pentanoic acid
EINECS	Contact for details

Quality Control

Our L-Leucine-D3-n-Fmoc (Methyl-D3) is manufactured under strict quality control protocols to ensure the highest isotopic and chemical purity required for sensitive analytical applications. Each batch is supported by a comprehensive Certificate of Analysis (COA) detailing key parameters such as isotopic enrichment (D%), chemical purity (by HPLC), and chiral purity. We adhere to cGMP principles where applicable to support our clients in regulated research environments.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a freezer. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability. Allow the sealed container to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Purity (D%)	≥ 98 atom % D
Chiral Purity (HPLC)	≥ 99.0% (L-isomer)
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.