Description

(1S)-n-[5-[(4-Mercaptobutanoyl)Amino]-1-Carboxypentyl]Iminodiacetic Acid (Hs-Nta) is a specialized, high-purity chelating agent and biochemical building block designed for advanced research and manufacturing processes. This compound is valued for its unique bifunctional structure, which combines a strong metal-chelating nitrilotriacetic acid (NTA) moiety with a reactive thiol (mercapto) group, enabling precise conjugation and immobilization strategies. It is essential for scientists and engineers in bioconjugation, proteomics, and the development of diagnostic assays and affinity chromatography media, where controlled surface functionalization and metal ion coordination are critical.

Application

• Bioconjugation and Protein Labeling: The thiol-reactive group allows for site-specific coupling to cysteine residues in proteins and peptides for creating labeled probes or antibody-drug conjugates (ADCs).
• Affinity Chromatography Media: Serves as a key ligand for immobilizing metal ions (e.g., Ni²⁺, Co²⁺) on solid supports to create resins for the purification of histidine-tagged recombinant proteins (IMAC).
• Surface Functionalization: Used to modify gold surfaces, nanoparticles, or biosensor chips via the thiol group, presenting a chelating NTA surface for controlled capture of metal-binding molecules.
• Diagnostic Assay Development: Employed in the fabrication of diagnostic platforms and microarrays where stable, oriented immobilization of biomolecules is required.
• Chemical Biology Research: A valuable tool for studying protein-protein interactions, metal ion trafficking, and developing novel bioanalytical methods.
• Precursor for Specialty Ligands: Acts as an intermediate in the synthesis of more complex chelators and cross-linking agents for therapeutic and diagnostic applications.

Basic Information

Product Name	(1S)-n-[5-[(4-Mercaptobutanoyl)Amino]-1-Carboxypentyl]Iminodiacetic Acid (Hs-Nta)
CAS No.	538328-49-3
Molecular Formula	C₁₄H₂₄N₂O₇S
Molecular Weight	364.41 g/mol
Synonyms	HS-NTA; Thiolated NTA; Nα-(4-Mercaptobutyryl)-L-lysine-Nω,Nω-bis(carboxymethyl); Mercaptosuccinyl-L-lysine-N,N-diacetic Acid (common naming variant); (S)-2-(4-((4-Mercaptobutanamido)methyl)-1-carboxybutylamino)diacetic Acid; N6-[(4-Mercapto-1-oxobutyl)]-N2,N2-bis(carboxymethyl)-L-lysine; A thiol-functionalized nitrilotriacetic acid derivative; NTA-Thiol.
EINECS	Contact for details

Quality Control

Our (1S)-n-[5-[(4-Mercaptobutanoyl)Amino]-1-Carboxypentyl]Iminodiacetic Acid (Hs-Nta) is manufactured under strict quality management systems to ensure batch-to-batch consistency and high purity suitable for research and bioconjugation applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. Our quality protocols are designed to meet the stringent requirements of GMP/GLP environments and advanced life science research.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (e.g., nitrogen or argon) for long-term stability, as the compound is easily oxidized and light-sensitive. For short-term use, store desiccated at 2-8°C. Allow the container to reach room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 2.0%
Free Thiol Content (Titration)	≥ 85% of theoretical
Heavy Metals (as Pb)	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.