Description

L-Methionine-D3-n-Fmoc (S-Methyl-D3) is a deuterium-labeled, N-Fmoc protected derivative of the essential amino acid methionine, specifically designed for advanced research and analytical applications. This high-purity, isotopically labeled compound serves as a critical internal standard and building block in mass spectrometry-based proteomics and metabolic studies, ensuring unparalleled accuracy in quantification and traceability. It is primarily utilized by pharmaceutical R&D teams, contract research organizations (CROs), and academic laboratories engaged in drug metabolism and pharmacokinetics (DMPK) research, peptide synthesis, and biomarker discovery.

Application

• Internal Standard for LC-MS/MS: Essential for the precise and accurate quantification of methionine and related metabolites in complex biological matrices like plasma, urine, and tissue homogenates.
• Stable Isotope Labeling in Proteomics (SILAC): Used in metabolic labeling strategies to study protein dynamics, synthesis rates, and post-translational modifications in cell culture and animal models.
• Peptide Synthesis & Medicinal Chemistry: Acts as a deuterium-labeled, Fmoc-protected building block for the solid-phase synthesis of isotopically labeled peptides for use as probes, standards, or in structure-activity relationship (SAR) studies.
• Drug Metabolism & Pharmacokinetics (DMPK): Critical in the development and validation of bioanalytical methods to track the absorption, distribution, metabolism, and excretion (ADME) of drug candidates containing methionine motifs.
• Biomarker Research: Enables the reliable measurement of methionine cycle metabolites (e.g., homocysteine, S-adenosylmethionine) in clinical and preclinical studies investigating diseases like cancer, cardiovascular disorders, and neurological conditions.
• NMR Spectroscopy: Serves as a useful tool in nuclear magnetic resonance studies due to the distinct signal of deuterium atoms, aiding in structural elucidation and dynamics analysis.

Basic Information

Product Name	L-Methionine-D3-n-Fmoc (S-Methyl-D3)
CAS No.	502692-58-2
Molecular Formula	C₂₁H₂₀D₃NO₄S
Molecular Weight	~381.48 g/mol
Synonyms	Fmoc-L-methionine(S-methyl-d3); N-[(9H-Fluoren-9-ylmethoxy)carbonyl]-L-methionine(S-methyl-d3); (2S)-2-[(9H-Fluoren-9-ylmethoxy)carbonyl]amino-4-(methyl-d3)sulfanylbutanoic acid; Fmoc-Met(S-CD3)-OH; Fmoc-L-Met(S-CD3)-OH; Nα-Fmoc-L-methionine(S-methyl-d3); L-Methionine-D3, N-Fmoc derivative (S-methyl-D3)
EINECS	Contact for details

Quality Control

Every batch of L-Methionine-D3-n-Fmoc (S-Methyl-D3) is manufactured and tested under strict quality management systems to ensure identity, purity, and isotopic enrichment meet the exacting standards required for analytical and synthetic applications. We provide comprehensive Certificates of Analysis (COA) with each shipment, detailing key parameters such as isotopic purity (D-enrichment), chemical purity (HPLC), and specific rotation. Our quality commitment aligns with the needs of cGMP-compliant research and development.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a freezer. This compound is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥98.0%
Isotopic Purity (D-enrichment)	≥98 atom % D
Specific Rotation	Contact for details
Water Content (KF)	≤1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.