Description

3-Hydroxy-3-Methyl-D3-Pentanedioic Acid is a deuterium-labeled derivative of 3-hydroxy-3-methylglutaric acid, a key intermediate in the biosynthesis of cholesterol and other isoprenoids. This compound matters for its critical role as a stable isotope-labeled internal standard in quantitative mass spectrometry, ensuring unparalleled accuracy in metabolic and pharmacokinetic studies. It is primarily needed by pharmaceutical R&D laboratories, clinical research organizations, and analytical chemistry facilities engaged in lipid metabolism research, drug development, and biomarker discovery.

Application

• Quantitative Mass Spectrometry: Serves as a crucial stable isotope-labeled internal standard (SIL-IS) for the precise quantification of endogenous 3-hydroxy-3-methylglutaric acid and related metabolites in biological matrices.
• Metabolic Pathway Tracing: Used in in vitro and in vivo studies to elucidate the mevalonate pathway and cholesterol biosynthesis dynamics using deuterium tracing techniques.
• Pharmacokinetic/Pharmacodynamic (PK/PD) Research: Enables accurate measurement of drug-induced changes in metabolic flux within the sterol synthesis pathway during preclinical and clinical trials.
• Biomarker Analysis & Clinical Diagnostics: Facilitates the development of highly specific and sensitive LC-MS/MS assays for diagnosing inborn errors of metabolism, such as HMG-CoA lyase deficiency.
• Pharmaceutical Impurity Profiling: Acts as a reference standard in the analysis and control of potential genotoxic impurities in statin drugs and other therapeutics targeting the HMG-CoA reductase enzyme.
• Academic & Institutional Research: Supports fundamental biochemical research into isoprenoid biosynthesis, enzyme kinetics, and metabolic regulation.

Basic Information

Product Name	3-Hydroxy-3-Methyl-D3-Pentanedioic Acid
CAS No.	59060-36-5
Molecular Formula	C6H7D3O5
Molecular Weight	165.15 g/mol
Synonyms	3-Hydroxy-3-methylglutaric acid-d3; 3-Hydroxy-3-methyl[5,5,5-2H3]pentanedioic acid; HMG-d3; Deuterated 3-hydroxy-3-methylglutaric acid; (3R/S)-3-Hydroxy-3-methylpentanedioic-5,5,5-d3 acid; 3-Hydroxy-3-methyl-D3-glutaric acid; 3-Methyl-3-hydroxyglutaric acid-d3; Stable Isotope Labeled HMG
EINECS	Contact for details

Quality Control

Every batch of 3-Hydroxy-3-Methyl-D3-Pentanedioic Acid is manufactured and analyzed under strict quality systems. We guarantee high chemical and isotopic purity, supported by comprehensive analytical data including NMR (1H, 13C), mass spectrometry, and HPLC. Certificates of Analysis (COA) detailing batch-specific results for identity, purity, isotopic enrichment, and residual solvents are provided and traceable to relevant standards for analytical and research use.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive); ensure the container is sealed under inert atmosphere after each use to maintain stability and prevent degradation.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Enrichment	≥ 98 atom % D
Chemical Purity (NMR)	≥ 97%
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.