Description

5-Maleimido Valeric Acid is a heterobifunctional crosslinking agent featuring a maleimide group and a carboxylic acid group, separated by a five-carbon spacer. This compound is essential for creating stable, site-specific conjugations between thiol-containing molecules and amine-containing molecules under mild conditions. It is a critical reagent for researchers and manufacturers in bioconjugation, antibody-drug conjugate (ADC) development, protein labeling, and surface functionalization.

Application

• Antibody-Drug Conjugate (ADC) Linker: A key building block for constructing cleavable or non-cleavable linkers that connect cytotoxic payloads to monoclonal antibodies.
• Protein and Peptide Modification: Used to introduce maleimide functional groups onto proteins or peptides via their amine groups (e.g., lysine residues) for subsequent thiol coupling.
• Surface Functionalization: Immobilization of biomolecules (like proteins or oligonucleotides) onto amine-coated surfaces, chips, or nanoparticles via the carboxylic acid group.
• Polymer Chemistry: Synthesis of functional polymers and hydrogels with reactive maleimide handles for biomedical and material science applications.
• Diagnostic Reagent Synthesis: Conjugation of enzymes, fluorophores, or other detection moieties to antibodies or other targeting molecules for assay development.
• Biomaterial Engineering: Creating crosslinked networks or modifying biomaterials to control cell adhesion, drug release, or mechanical properties.

Basic Information

Product Name	5-Maleimido Valeric Acid
CAS No.	57078-99-6
Molecular Formula	C9H11NO4
Molecular Weight	197.19 g/mol
Synonyms	5-Maleimidopentanoic Acid; 6-Maleimidohexanoic Acid (common misnomer); N-(5-Carboxypentyl)maleimide; 4-((2,5-Dioxo-2,5-dihydro-1H-pyrrol-1-yl)pentanoic acid; Maleimidocaproic Acid (common name); γ-Maleimidobutyric Acid (related analog); N-Succinimidyl 4-Maleimidobutyrate precursor; EMCS (a related NHS ester); SMPB precursor
EINECS	Contact for details

Quality Control

Our 5-Maleimido Valeric Acid is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation by NMR and IR spectroscopy, to ensure it meets the high standards required for sensitive bioconjugation applications. A detailed Certificate of Analysis (COA) is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a freezer at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and should be allowed to equilibrate to room temperature in a dry environment before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Melting Point	Contact for details
Water Content (KF)	≤ 0.5%
Solubility	Soluble in DMF, DMSO, Acetone; Slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.