Description

4-Maleimidobutyric Acid is a heterobifunctional crosslinking agent featuring a maleimide group and a carboxylic acid group, separated by a flexible butyric acid spacer. This molecular architecture is critical for its primary role in bioconjugation, enabling the stable and site-specific coupling of biomolecules. It is an essential building block for researchers and manufacturers in the pharmaceutical and biotechnology sectors, particularly in the development of antibody-drug conjugates (ADCs), protein labeling, and surface immobilization. The compound's reactivity profile allows for the creation of stable thioether bonds with sulfhydryl groups under mild conditions.

Application

• Antibody-Drug Conjugate (ADC) Linker Synthesis: Serves as a key intermediate for constructing cleavable or non-cleavable linkers that connect cytotoxic payloads to monoclonal antibodies.
• Protein and Peptide Modification: Used for site-specific labeling of cysteine residues in proteins and peptides with fluorescent dyes, biotin, or other functional probes.
• Surface Functionalization: Enables the covalent immobilization of thiol-containing biomolecules onto carboxylated surfaces, chips, or nanoparticles for biosensor and diagnostic assay development.
• Polymer Conjugation: Facilitates the grafting of peptides or proteins onto polymeric backbones to create advanced biomaterials and drug delivery systems.
• Enzyme Stabilization: Employed in crosslinking enzymes to enhance their thermal stability and operational lifespan in industrial biocatalysis.
• Hydrogel Formation: Acts as a crosslinker in the synthesis of hydrogels with tailored mechanical properties and bioactive functionalities for tissue engineering.

Basic Information

Product Name	4-Maleimidobutyric Acid
CAS No.	57078-98-5
Molecular Formula	C8H9NO4
Molecular Weight	183.16 g/mol
Synonyms	4-(2,5-Dihydro-2,5-dioxo-1H-pyrrol-1-yl)butanoic Acid; GMBS (N-(γ-Maleimidobutyryloxy)succinimide ester precursor); Maleimidobutyric Acid; 1-(3-Carboxypropyl)-1H-pyrrole-2,5-dione; 4-Maleimidobutanoic Acid; γ-Maleimidobutyric Acid; N-Maleoyl-γ-aminobutyric Acid
EINECS	Contact for details

Quality Control

Our 4-Maleimidobutyric Acid is manufactured under strict quality management systems. Every batch undergoes comprehensive analytical testing to ensure high purity and consistency, meeting the stringent requirements for research and bioconjugation applications. Certificates of Analysis (COA) detailing purity, identity, and impurity profiles are provided with each shipment. We support compliance with relevant guidelines for advanced pharmaceutical intermediates.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C (refrigerated) or as specified on the label. The product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to minimize exposure to atmospheric moisture. For long-term storage, consider under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Melting Point	Contact for details
Water Content (KF)	≤ 0.5%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.