Description

D-[4-2H]Glucose is a stable isotope-labeled analog of D-glucose, specifically deuterated at the 4-position. This compound is an essential tracer in metabolic research, enabling precise tracking and quantification of glucose utilization and metabolic flux in biological systems. It is primarily utilized by researchers and manufacturers in the pharmaceutical, biotechnology, and life science sectors for advanced studies in metabolism, drug development, and diagnostic assay development.

Application

• Metabolic Pathway Analysis: Used as a stable isotope tracer in in vitro and in vivo studies to elucidate glycolysis, gluconeogenesis, and the pentose phosphate pathway.
• Drug Metabolism & Pharmacokinetics (DMPK): Serves as an internal standard in LC-MS/MS and GC-MS methods for the accurate bioanalysis of glucose and related metabolites in preclinical and clinical studies.
• Biomedical Research: Employed in NMR and mass spectrometry-based metabolomics to investigate metabolic disorders such as diabetes, cancer, and obesity.
• Diagnostic Reagent Manufacturing: A key component in the formulation of calibration standards and controls for clinical diagnostic assays measuring glucose metabolism.
• Isotope Dilution Mass Spectrometry (IDMS): Provides a definitive reference material for the highly accurate quantification of natural glucose levels in complex biological matrices.
• Enzyme Mechanism Studies: Utilized to study the kinetics and mechanisms of enzymes like hexokinase and glucose-6-phosphate dehydrogenase using deuterium isotope effects.

Basic Information

Product Name	D-[4-2H]Glucose
CAS No.	56570-89-9
Molecular Formula	C6H11DO6
Molecular Weight	181.16 g/mol
Synonyms	4-Deuterio-D-glucose; D-Glucose-4-d; Deuterated D-glucose (4-2H); 4-2H-D-Glucose; (4R)-4-Deuterio-D-glucose; D-Glucose (4-2H); Stable Isotope Labeled D-Glucose; Glucose-4-d1
EINECS	Contact for details

Quality Control

Every batch of D-[4-2H]Glucose is manufactured and tested under strict quality management systems. We guarantee high chemical and isotopic purity, with comprehensive testing including assay, isotopic enrichment (D%), and specific optical rotation. Certificates of Analysis (COA) detailing all specifications and test results are provided to ensure traceability and compliance with your research and production requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This material is hygroscopic (moisture-sensitive) and must be kept under anhydrous conditions. For long-term storage, consider storing under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0% (anhydrous basis)
Isotopic Enrichment	≥ 98 atom % D
Specific Optical Rotation	+52.5° to +53.5° (c=10, H2O)
Water Content (KF)	≤ 1.0 %
Residue on Ignition	≤ 0.1 %

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.