Description

4,4-D2-Cyclophosphamide is a deuterated analog of the widely used chemotherapeutic agent cyclophosphamide. This compound is of significant value for pharmaceutical research and development, particularly in drug metabolism and pharmacokinetic (DMPK) studies. It serves as a critical internal standard in mass spectrometry-based bioanalytical assays, enabling precise quantification and reducing matrix effects. Researchers in pharmaceutical development, contract research organizations (CROs), and academic institutions require this high-purity standard to ensure the accuracy and reliability of their analytical data.

Application

• Internal Standard for LC-MS/MS: Used as a stable isotope-labeled internal standard for the accurate quantification of cyclophosphamide and its metabolites in biological matrices like plasma, serum, and urine.
• Drug Metabolism Studies (DMPK): Essential for tracing metabolic pathways, studying isotope effects, and understanding the pharmacokinetic profile of cyclophosphamide in preclinical and clinical research.
• Bioanalytical Method Development: Critical for developing and validating robust, sensitive, and specific analytical methods in compliance with regulatory guidelines (e.g., FDA, EMA).
• Pharmaceutical R&D: Supports drug discovery and development programs focused on oncology and immunosuppressive therapies.
• Clinical Research: Employed in therapeutic drug monitoring (TDM) and clinical trial sample analysis to ensure data integrity.
• Reference Material: Serves as a certified reference material (CRM) for quality control and assurance in analytical laboratories.

Basic Information

Product Name	4,4-D2-Cyclophosphamide
CAS No.	55836-73-2
Molecular Formula	C7H13D2Cl2N2O2P
Molecular Weight	264.09 g/mol (deuterated form)
Synonyms	Cyclophosphamide-d2; 2-[Bis(2-chloroethyl)amino]tetrahydro-4,4-d2-2H-1,3,2-oxazaphosphorine 2-oxide; Deuterated Cyclophosphamide; Cyclophosphamide (4,4-D2); NSC-26271-d2; CP-d2; Endoxan-d2; Cytoxan-d2
EINECS	Contact for details

Quality Control

Our 4,4-D2-Cyclophosphamide is manufactured under strict quality control protocols to ensure the highest standards of purity and isotopic enrichment, which are critical for its use as an analytical standard. Each batch is accompanied by a comprehensive Certificate of Analysis (COA) detailing specifications for isotopic purity, chemical purity, and identity confirmation. We adhere to relevant industry best practices to support research requiring reliable and consistent performance in sensitive applications like mass spectrometry.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive) and light-sensitive; therefore, containers must be kept tightly sealed in a desiccated environment away from direct light exposure to maintain stability and purity.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Purity (D-content)	≥ 98 atom % D
Chemical Purity (NMR)	Conforms
Residual Solvents (GC)	Meets ICH guidelines
Water Content (KF)	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.