Description

3-O,4-o-Bis(Trimethylsilyl)-2-Deoxy-D-Erythro-Pentose 5-[n-Methoxyimidophosphoric Acid Bis(Trimethylsilyl)] Ester is a sophisticated, fully protected nucleoside phosphoramidite derivative, a critical building block in automated oligonucleotide synthesis. This compound matters because it enables the precise and efficient incorporation of modified 2'-deoxyribose units into synthetic DNA strands, allowing researchers to create custom sequences with specific functionalities. It is essential for pharmaceutical R&D, academic research, and biotechnology companies focused on developing antisense oligonucleotides, siRNA, aptamers, and other advanced nucleic acid therapeutics.

Application

• Solid-Phase Oligonucleotide Synthesis: As a phosphoramidite monomer for the automated synthesis of DNA strands containing specific, protected sugar modifications.
• Antisense & siRNA Therapeutic Development: A key raw material for producing research-scale and pre-clinical batches of therapeutic oligonucleotides targeting genetic diseases.
• Diagnostic Probe Manufacture: Used in the synthesis of labeled DNA probes for diagnostic assays, including PCR and hybridization techniques.
• Aptamer Research: Enables the construction of synthetic nucleic acid libraries (SELEX) for selecting high-affinity binding molecules.
• Chemical Biology Studies: Facilitates the site-specific introduction of modifications into DNA to study structure-activity relationships and biomolecular interactions.
• Process Development for GMP Manufacturing: Serves as a reference standard and starting material for scaling up oligonucleotide active pharmaceutical ingredient (API) production under cGMP guidelines.

Basic Information

Product Name	3-O,4-o-Bis(Trimethylsilyl)-2-Deoxy-D-Erythro-Pentose 5-[n-Methoxyimidophosphoric Acid Bis(Trimethylsilyl)] Ester
CAS No.	55517-63-0
Molecular Formula	C₂₀H₄₉N₂O₆PSi₄
Molecular Weight	557.94 g/mol
Synonyms	2'-Deoxy-3',4'-bis-O-(trimethylsilyl)ribofuranosyl 5'-[N-Methoxy-bis(trimethylsilyl)phosphoramidite]; 5'-O-[Bis(trimethylsilyl)amino-methoxyphosphinyl]-2'-deoxy-3',4'-bis-O-(trimethylsilyl)ribofuranose; Protected 2'-Deoxyribose Phosphoramidite; TMS-Protected Deoxyribose Phosphoramidite; Nucleoside Phosphoramidite Derivative; DNA Synthesis Building Block; Oligonucleotide Synthesis Intermediate
EINECS	Contact for details

Quality Control

Every batch of this high-value phosphoramidite is subjected to rigorous analytical testing to ensure identity, purity, and reactivity suitable for sensitive oligonucleotide synthesis. Quality is verified via HPLC, ¹H/³¹P NMR, and mass spectrometry. A comprehensive Certificate of Analysis (COA) detailing purity, moisture content, and performance in coupling tests is provided with each shipment. Our quality system supports development under ISO 9001 standards, with options available for synthesis in facilities operating under GMP principles for advanced therapeutic applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a dedicated freezer. This compound is hygroscopic (moisture-sensitive) and must be handled in a dry, controlled environment. Allow the sealed vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	Colorless to pale yellow viscous liquid or solid
Identification (IR)	Conforms to structure
Identification (³¹P NMR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 0.5%
Solubility	Soluble in anhydrous acetonitrile, THF, dichloromethane

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.