Description

Cholic Acid-2,2,3,4,4-D is a high-purity, deuterium-labeled derivative of cholic acid, a primary bile acid. This stable isotope-labeled compound is essential for advanced analytical research, providing critical internal standards for precise quantification in mass spectrometry. It is primarily utilized by research institutions, pharmaceutical developers, and diagnostic manufacturers engaged in metabolomics, lipidomics, and drug metabolism studies.

Application

• Internal Standard for LC-MS/MS: Serves as a critical quantitative reference standard in liquid chromatography-tandem mass spectrometry for analyzing endogenous bile acids.
• Metabolomics Research: Enables accurate tracking and measurement of bile acid pathways and metabolic flux in biological systems.
• Drug Development & Pharmacokinetics: Used in ADME (Absorption, Distribution, Metabolism, Excretion) studies to monitor the metabolism of drug candidates affecting hepatic pathways.
• Clinical Diagnostics: Facilitates the development of high-precision diagnostic assays for liver function disorders, cholestasis, and other metabolic diseases.
• Stable Isotope Labeling Studies: Acts as a tracer in biomedical research to elucidate the biosynthesis and enterohepatic circulation of bile acids.

Basic Information

Product Name	Cholic Acid-2,2,3,4,4-D
CAS No.	53007-09-3
Molecular Formula	C₂₄H₃₅D₅O₅
Molecular Weight	413.59 g/mol (approx.)
Synonyms	(3α,5β,7α,12α)-3,7,12-Trihydroxy-24-cholan-23-oic-2,2,3,4,4-d5 Acid; Deuterated Cholic Acid; Cholic Acid-d5; 3α,7α,12α-Trihydroxy-5β-cholan-24-oic-2,2,3,4,4-d5 Acid; Cholalic Acid-d5; Cholate-d5; 5β-Cholan-24-oic-3α,7α,12α-triol-d5 Acid
EINECS	Contact for details

Quality Control

Every batch of Cholic Acid-2,2,3,4,4-D is manufactured under controlled conditions and undergoes rigorous analytical testing to ensure exceptional isotopic purity and chemical identity. Our quality commitment aligns with the stringent requirements for research-grade stable isotope-labeled compounds. A comprehensive Certificate of Analysis (COA) detailing isotopic enrichment (D%), chemical purity (by HPLC), and spectroscopic confirmation is provided with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This material is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to minimize exposure to atmospheric moisture.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Isotopic Purity (D%)	≥ 98 atom % D
Chemical Purity (HPLC)	≥ 98.0%
Specific Optical Rotation	Contact for details
Residual Solvents (GC)	Complies with ICH guidelines
Water Content (KF)	≤ 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.