Description

Deoxycholic Acid-D5 is a deuterium-labeled analog of the primary bile acid, deoxycholic acid, where five hydrogen atoms are replaced with deuterium. This stable isotope-labeled compound is essential for use as an internal standard in quantitative mass spectrometry (MS) and liquid chromatography-mass spectrometry (LC-MS) analyses, ensuring unparalleled accuracy and reproducibility in complex biological matrices. It is a critical tool for researchers and quality control laboratories in the pharmaceutical, biotechnology, and clinical diagnostics sectors who require precise measurement of bile acid metabolism, pharmacokinetic studies, and biomarker discovery.

Application

• Quantitative Mass Spectrometry Internal Standard: Serves as a critical reference compound for the accurate quantification of endogenous deoxycholic acid and related bile acids in biological samples (e.g., serum, plasma, urine, tissue) via LC-MS/MS and GC-MS.
• Drug Metabolism and Pharmacokinetics (DMPK) Studies: Used in pharmaceutical R&D to trace the absorption, distribution, metabolism, and excretion (ADME) profiles of drug candidates and to study bile acid-related drug interactions.
• Clinical Diagnostics and Biomarker Research: Enables precise measurement of bile acid profiles for research into liver diseases (cholestasis, cirrhosis), gastrointestinal disorders, and metabolic conditions.
• Stable Isotope Dilution Assays: Fundamental for high-precision assays where correcting for matrix effects and instrument variability is paramount for reliable data.
• Analytical Method Development and Validation: Used to develop, optimize, and validate robust bioanalytical methods in compliance with regulatory guidelines (e.g., FDA, EMA).
• Nutritional and Microbiome Research: Facilitates studies on gut microbiome-host interactions by accurately quantifying microbial-derived secondary bile acids.

Basic Information

Product Name	Deoxycholic Acid-D5
CAS No.	52840-14-9
Molecular Formula	C24H35D5O4
Molecular Weight	397.59 g/mol
Synonyms	3α,12α-Dihydroxy-5β-cholan-24-oic-2,2,3,4,4-d5 acid; Deuterated Deoxycholic Acid; (5β)-3α,12α-Dihydroxycholan-24-oic-2,2,3,4,4-d5 acid; DCA-d5; 5β-Cholan-24-oic-2,2,3,4,4-d5 acid, 3α,12α-dihydroxy-, (5β)-; Desoxycholic Acid-d5; Deoxycholic Acid (2,2,3,4,4-d5)
EINECS	Contact for details

Quality Control

Our Deoxycholic Acid-D5 is manufactured under strict quality systems to ensure the high chemical and isotopic purity required for sensitive analytical applications. Each batch undergoes comprehensive testing, including NMR (¹H & ²H) and Mass Spectrometry to confirm deuteration level and positional specificity, alongside HPLC for chemical purity assessment. A detailed Certificate of Analysis (COA) is provided, documenting the isotopic purity (typically ≥98 atom % D), chemical purity (≥95%), and absence of critical unlabeled impurities. Our quality commitment supports compliance with ISO 9001 standards and the rigorous demands of GLP/GMP environments.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C (refrigerated) or as indicated on the label or COA. This product is hygroscopic (moisture-sensitive). Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Isotopic Purity (NMR/MS)	≥ 98 atom % D
Chemical Purity (Titration)	Contact for details
Loss on Drying	≤ 2.0 %
Residue on Ignition	≤ 0.1 %

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.