Description

Thymolphthalein Monophosphate 2-Amino-2-Methyl-1,3-Propanediol Salt is a high-purity chromogenic substrate salt, specifically designed for sensitive enzymatic detection and quantification. This compound is critical for biochemical research and diagnostic applications where precise and reliable colorimetric analysis is required. It is primarily utilized by manufacturers of diagnostic kits, research laboratories in life sciences, and quality control departments in the pharmaceutical industry for assays involving alkaline phosphatase and other phosphatases.

Application

• Diagnostic Reagent Manufacturing: As a key chromogenic substrate in clinical chemistry for the detection of alkaline phosphatase activity in ELISA, immunoblotting, and automated analyzer systems.
• Biochemical Research: For studying enzyme kinetics, inhibition, and activity of phosphatases in various experimental models.
• Pharmaceutical Quality Control: Used in assay development and validation to ensure the potency and purity of enzyme-based pharmaceuticals.
• Life Science Assays: Employed in high-throughput screening (HTS) platforms and histochemical staining to visualize enzymatic activity in cells and tissues.
• Food & Environmental Testing: Potential application in biosensors and test kits for detecting bacterial contamination or phosphatase activity as an indicator.

Basic Information

Product Name	Thymolphthalein Monophosphate 2-Amino-2-Methyl-1,3-Propanediol Salt
CAS No.	52279-66-0
Molecular Formula	C31H38N2O10P
Molecular Weight	620.62 g/mol
Synonyms	Thymolphthalein monophosphate AMPD salt; Thymolphthalein phosphate AMP salt; 5',5''-Diisopropyl-2',2''-dimethylphenolphthalein phosphate 2-amino-2-methyl-1,3-propanediol salt; AMP-Thymolphthalein monophosphate; TMP-AMPD; Alkaline phosphatase substrate (thymolphthalein based); Diagnostic chromogen; 2-Amino-2-methylpropane-1,3-diol thymolphthalein monophosphate
EINECS	Contact for details

Quality Control

Our Thymolphthalein Monophosphate AMPD Salt is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the stringent requirements for diagnostic and research applications. A Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment to guarantee traceability and consistency.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). The product is light-sensitive; prolonged exposure to light should be avoided to maintain stability and performance.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in water, DMSO
Heavy Metals	< 20 ppm
Residual Solvents	Complies with ICH guidelines
Performance (Enzymatic)	Conforms to reference standard

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.