Description

p-Nitrophenyl-B-D-Thiofucopyranoside is a specialized chromogenic substrate used in enzymatic assays. This compound is critical for the detection and quantification of specific glycosidase enzymes, particularly fucosidases, due to its high sensitivity and specificity. It is an essential reagent for researchers and manufacturers in the fields of biochemical research, diagnostic kit development, and pharmaceutical quality control.

Application

• Enzymatic Substrate for Fucosidase Activity Assays: Used as a chromogenic substrate to measure α-L-fucosidase activity in research and clinical diagnostics.
• Diagnostic Reagent Manufacturing: A key component in the production of diagnostic kits for genetic disorders like fucosidosis and other lysosomal storage diseases.
• Biochemical Research Tool: Employed in enzyme kinetics studies, inhibitor screening, and characterization of glycosidase enzyme mechanisms.
• Pharmaceutical Quality Control: Utilized in purity and activity testing of enzyme-based biopharmaceuticals.
• Microbiology and Cell Biology: Applied in studies of carbohydrate metabolism and glycoprotein processing in cells and microorganisms.

Basic Information

Product Name	p-Nitrophenyl-B-D-Thiofucopyranoside
CAS No.	51885-02-0
Molecular Formula	C12H15NO7S
Molecular Weight	317.31 g/mol
Synonyms	4-Nitrophenyl β-D-thiofucopyranoside; p-Nitrophenyl β-D-thiofucoside; PNPTF; 4-Nitrophenyl 6-deoxy-β-D-thiogalactopyranoside; β-D-Thiofucopyranoside, 4-nitrophenyl; 1-Thio-β-D-fucopyranoside, 4-nitrophenyl; 4-Nitrophenyl 1-thio-β-D-fucopyranoside
EINECS	Contact for details

Quality Control

Our p-Nitrophenyl-B-D-Thiofucopyranoside is manufactured under strict quality systems to ensure batch-to-batch consistency and high purity suitable for sensitive biochemical applications. Each lot undergoes comprehensive analytical testing including HPLC for purity, identity confirmation by spectroscopic methods (IR, NMR), and specific activity validation. Certificates of Analysis (COA) detailing all specifications are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and should be handled in a dry environment to prevent degradation. For long-term storage, consider desiccation.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Specific Rotation	Contact for details
Melting Point	Contact for details
Solubility	Soluble in DMSO, DMF; sparingly soluble in water
Biological Activity	Contact for details

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.