Description

Dimethylmalonimidate is a versatile bifunctional imidoester crosslinking reagent primarily used for protein modification and immobilization. Its high reactivity towards primary amines under mild, aqueous conditions makes it an essential tool for creating stable conjugates while preserving biological activity. This compound is critical for researchers and manufacturers in bioconjugation, diagnostics development, and affinity chromatography, where precise and stable covalent linkages are required.

Application

• Protein-Protein Crosslinking: For creating stable antibody-enzyme or protein-polymer conjugates used in immunoassays and therapeutic agents.
• Affinity Chromatography Ligand Immobilization: Covalently coupling proteins, antibodies, or other ligands to solid supports like agarose or sepharose beads.
• Diagnostic Kit Manufacturing: As a key reagent for labeling and stabilizing components in ELISA and lateral flow assay platforms.
• Cell Surface Labeling: For studying membrane proteins and receptors without penetrating the cell membrane.
• Peptide Synthesis & Modification: Acting as a spacer or linker in solid-phase peptide synthesis and peptide conjugate preparation.
• Biomaterial & Hydrogel Fabrication: Crosslinking amine-containing polymers to create scaffolds for tissue engineering and drug delivery systems.
• Nucleic Acid Research: For crosslinking proteins to nucleic acids in molecular biology studies.

Basic Information

Product Name	Dimethylmalonimidate
CAS No.	51534-05-5
Molecular Formula	C7H12N2O2
Molecular Weight	156.18 g/mol
Synonyms	Dimethyl Malonimidate; Dimethyl Malonimidate Dihydrochloride; Malonimidic Acid Dimethyl Ester; DMMI; NSC 102811; 2,2-Dimethoxy-N,N'-dimethylmalonimidamide; Propanedimidic Acid, Dimethyl Ester
EINECS	Contact for details

Quality Control

Our Dimethylmalonimidate is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR for structural confirmation, and Karl Fischer titration for moisture content. We provide a detailed Certificate of Analysis (COA) with every shipment, ensuring traceability and compliance with your research or production specifications. Our quality commitment supports applications in GMP-sensitive diagnostic and biopharmaceutical development.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C (refrigerated) or as specified on the label. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability and reactivity. Allow the sealed container to reach room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Melting Point	Contact for details
Solubility	Soluble in water, methanol, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.