Description

Methyl Isolithocholate-D7 is a deuterium-labeled derivative of the bile acid methyl ester, specifically designed for use as an internal standard in quantitative analytical methods. This high-purity compound is essential for ensuring accuracy and precision in mass spectrometry-based analyses, where it corrects for variations in sample preparation and instrument response. It is a critical tool for researchers and quality control laboratories in the pharmaceutical, clinical diagnostics, and metabolomics fields who require reliable isotopic standards for the study of bile acid metabolism and related biochemical pathways.

Application

• Internal Standard for LC-MS/MS: Primary use as a quantitative reference standard in liquid chromatography-tandem mass spectrometry for the analysis of bile acids and their conjugates.
• Metabolomics Research: Enables precise quantification of endogenous lithocholic acid derivatives in complex biological samples like serum, urine, and tissue homogenates.
• Drug Metabolism and Pharmacokinetics (DMPK): Used in studies to track the metabolism of drug candidates that affect or are processed by bile acid pathways.
• Clinical Diagnostic Assay Development: Serves as a critical component in the development and validation of diagnostic tests for liver function disorders, cholestasis, and other metabolic diseases.
• Biomarker Discovery and Validation: Facilitates the accurate measurement of bile acid profiles as potential biomarkers for gastrointestinal and hepatic diseases.
• Stable Isotope Labeling Studies: Employed in tracer studies to investigate the dynamics and fluxes within the bile acid pool in vivo and in vitro.

Basic Information

Product Name	Methyl Isolithocholate-D7
CAS No.	5405-42-5
Molecular Formula	C25H35D7O4
Molecular Weight	409.62 g/mol (approx.)
Synonyms	3α-Hydroxy-5β-cholan-24-oic Acid Methyl Ester-d7; Methyl 3α-hydroxy-5β-cholan-24-oate-d7; Isolithocholic Acid Methyl Ester-d7; 3α-Hydroxy-5β-cholanoic Acid Methyl Ester-d7; Deuterated Methyl Isolithocholate; Methyl 3-Deuteriooxycholate-d6; (3α,5β)-3-Hydroxy-cholan-24-oic Acid Methyl Ester-d7
EINECS	Contact for details

Quality Control

Every batch of Methyl Isolithocholate-D7 is manufactured and analyzed under strict quality systems to ensure the high chemical purity and isotopic enrichment required for sensitive analytical applications. Our products undergo rigorous quality testing to ensure compliance with industry standards. Certificates of Analysis (COA) providing detailed specifications for identity, purity (by HPLC), isotopic purity (by MS), and solvent content are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below for long-term stability. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture condensation. The product is hygroscopic (moisture-sensitive).

Specification

Item	Specification
Appearance	White to off-white solid
Identification (IR)	Conforms to structure
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Purity	≥ 98.0 atom % D
Chemical Purity	≥ 95.0% (by NMR)
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.