Description

n-Hydroxymethylsuccinimide is a versatile bifunctional chemical building block that combines the reactivity of an N-hydroxysuccinimide (NHS) ester with an alcohol group. This unique structure makes it a critical intermediate for the synthesis of active esters, which are widely used in bioconjugation and crosslinking applications to create stable amide bonds under mild conditions. It is essential for researchers and manufacturers in the pharmaceutical, biotech, and advanced materials sectors who require reliable, high-purity reagents for peptide synthesis, protein labeling, and polymer modification.

Application

• Bioconjugation Reagent: A key intermediate for synthesizing NHS-activated esters used to label proteins, antibodies, and other biomolecules.
• Peptide Synthesis: Employed as an activating agent for carboxyl groups during solid-phase and solution-phase peptide coupling reactions.
• Polymer & Material Science: Used to functionalize polymers, surfaces, and nanoparticles with reactive ester groups for further chemical modification.
• Crosslinking Agent: Serves as a precursor for homo- and hetero-bifunctional crosslinkers in the development of hydrogels and biomaterials.
• Diagnostic Reagents: Integral in the manufacturing of enzyme conjugates and other components for immunoassay and diagnostic kits.
• Organic Synthesis Intermediate: A valuable synthon for constructing more complex molecules in medicinal chemistry and agrochemical research.

Basic Information

Product Name	n-Hydroxymethylsuccinimide
CAS No.	5146-68-9
Molecular Formula	C5H7NO3
Molecular Weight	129.11 g/mol
Synonyms	1-Hydroxymethyl-2,5-pyrrolidinedione; N-(Hydroxymethyl)succinimide; 1-(Hydroxymethyl)-2,5-pyrrolidinedione; Hydroxymethylsuccinimide; Succinimide, N-(hydroxymethyl)-; NSC 401122
EINECS	225-902-5

Quality Control

Our n-Hydroxymethylsuccinimide is manufactured under strict quality management systems. Every batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation by IR and NMR spectroscopy, to ensure it meets the high standards required for research and production applications. A comprehensive Certificate of Analysis (COA) detailing all specifications is provided with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry, well-ventilated area at controlled room temperature (15-25°C). This compound is hygroscopic (moisture-sensitive); keep the container tightly sealed to prevent absorption of atmospheric moisture.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Melting Point	118 - 122 °C
Loss on Drying	≤ 0.5%
Heavy Metals	≤ 10 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.