Description

Pyridoxine-D2 Hcl CAS NO 5027-82-7 is a deuterium-labeled isotopologue of Pyridoxine Hydrochloride (Vitamin B6), where two hydrogen atoms are replaced by deuterium. This stable isotope-labeled compound is essential for advanced analytical and research applications requiring precise molecular tracking and quantification. It is a critical material for pharmaceutical research, clinical diagnostics, and metabolic studies, serving scientists and quality control laboratories in the life sciences sector.

Application

• Internal Standard for Quantitative Analysis: Used as a stable isotope-labeled internal standard in LC-MS/MS and GC-MS methods for the accurate quantification of Vitamin B6 and its metabolites in complex biological matrices.
• Pharmaceutical Research & Development: Employed in drug metabolism and pharmacokinetics (DMPK) studies to trace the absorption, distribution, metabolism, and excretion (ADME) of pyridoxine and related compounds.
• Clinical Diagnostics: Facilitates the development of highly specific and sensitive diagnostic assays for measuring vitamin B6 status in human plasma, serum, and tissues.
• Nutritional and Metabolic Research: A vital tool for investigating vitamin B6-dependent enzymatic pathways, metabolic flux analysis, and nutritional bioavailability studies.
• Quality Control & Method Validation: Serves as a reference standard in pharmaceutical quality control laboratories to validate analytical methods for potency and impurity profiling of Vitamin B6 formulations.

Basic Information

Product Name	Pyridoxine-D2 Hcl
CAS No.	5027-82-7
Molecular Formula	C8H9D2ClNO3 • HCl
Molecular Weight	228.10 g/mol (for monoisotopic mass)
Synonyms	Vitamin B6-d2 Hydrochloride; Pyridoxine-4,5-d2 Hydrochloride; 5-Hydroxy-6-methyl-3,4-pyridinedimethanol-4,5-d2 Hydrochloride; 2-Methyl-3-hydroxy-4,5-bis(hydroxymethyl)pyridine-d2 Hydrochloride; Pyridoxol-d2 HCl; Deuterated Pyridoxine HCl; [4,5-D2]-Pyridoxine Hydrochloride
EINECS	Contact for details

Quality Control

Our Pyridoxine-D2 Hcl is manufactured under strict quality systems to ensure high chemical and isotopic purity, critical for research and analytical applications. Each batch is supported by a comprehensive Certificate of Analysis (COA) detailing parameters such as isotopic enrichment (Atom % D), chemical purity by HPLC, and identification by spectroscopic methods. We adhere to relevant standards for high-purity reference materials to ensure reliable and reproducible results in your sensitive applications.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive) and should be handled in a dry environment to prevent degradation. For long-term storage, consider desiccated conditions.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Enrichment	≥ 98.0 atom % D
Chemical Purity (by NMR)	Conforms
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.