Description

[5',5''-2H2]2'-Deoxyuridine is a deuterium-labeled isotopologue of the nucleoside 2'-deoxyuridine, a critical building block in nucleic acid chemistry. This stable isotope-labeled compound is essential for advanced research requiring precise tracking and quantification of metabolic pathways and nucleic acid synthesis. It is primarily utilized by researchers and manufacturers in the fields of pharmaceutical development, life sciences, and analytical chemistry for applications such as mass spectrometry-based assays and metabolic studies.

Application

• Internal Standard for Mass Spectrometry (MS): Serves as a critical reference standard in LC-MS and GC-MS analysis for the accurate quantification of unlabeled 2'-deoxyuridine and related metabolites in complex biological matrices.
• Metabolic Pathway Tracing: Used in pharmacokinetic (PK) and drug metabolism studies to track the incorporation and fate of nucleosides, aiding in the development of antiviral and anticancer therapeutics.
• Biochemical Research: Employed in enzymatic studies and in vitro assays to investigate the mechanisms of thymidylate synthase and other nucleotide-metabolizing enzymes.
• Deuterated Pharmaceutical Intermediate: Acts as a key starting material or intermediate in the synthesis of deuterated drug candidates, where deuterium incorporation can improve metabolic stability.
• NMR Spectroscopy: Provides a useful tool in nuclear magnetic resonance (NMR) spectroscopy due to the distinct properties of deuterium, aiding in structural elucidation and dynamics studies.

Basic Information

Product Name	[5',5''-2H2]2'-Deoxyuridine
CAS No.	478511-30-7
Molecular Formula	C9H10D2N2O5
Molecular Weight	230.21 g/mol
Synonyms	2'-Deoxy-5,5-dideuterouridine; 1-[(2R,4S,5R)-4-Hydroxy-5-(hydroxymethyl)oxolan-2-yl]-5-(2H2)pyrimidine-2,4-dione; dU-d2; Deuterated 2'-Deoxyuridine; 2'-Deoxyuridine-5,5-d2; 5,5-Dideutero-2'-deoxyuridine; Uridine, 2'-deoxy-5,5-d2-
EINECS	Contact for details

Quality Control

Every batch of [5',5''-2H2]2'-Deoxyuridine is produced and analyzed under strict quality protocols to ensure high isotopic purity and chemical integrity, meeting the stringent requirements of research and development. Our quality assurance includes comprehensive analytical testing via NMR, HPLC, and MS. A Certificate of Analysis (COA) detailing purity, isotopic enrichment, and analytical results is provided with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature (15-25°C) in a dry environment. For long-term storage, consider storing at -20°C under inert atmosphere to ensure maximum stability.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Enrichment	≥ 98 atom % D
Chemical Purity	≥ 95%
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.