Description

[5',5''-2H2]2'-Deoxyguanosine Monohydrate is a stable isotope-labeled nucleoside derivative, specifically deuterated at the 5' and 5'' positions. This compound is critical for ensuring accuracy and reliability in quantitative mass spectrometry-based analyses, where it serves as an internal standard to correct for variations in sample preparation and instrument response. It is an essential tool for researchers and quality control laboratories in the pharmaceutical, biotechnology, and diagnostic sectors, particularly those focused on metabolomics, pharmacokinetic studies, and nucleic acid research.

Application

• Internal Standard for LC-MS/MS: Used as a quantitative reference standard in liquid chromatography-tandem mass spectrometry for the accurate measurement of endogenous 2'-deoxyguanosine and related metabolites.
• Biomedical Research: Facilitates studies on DNA damage, repair mechanisms, and cellular metabolism by enabling precise tracking and quantification.
• Drug Metabolism and Pharmacokinetics (DMPK): Employed in the development and validation of bioanalytical methods to study the disposition of nucleoside-based pharmaceuticals.
• Clinical Diagnostics: Supports the development of high-precision assays for biomarkers related to oxidative stress, mitochondrial disorders, and cancer.
• Isotope Dilution Mass Spectrometry (IDMS): Serves as the labeled spike in IDMS protocols, which are considered a gold standard for achieving high metrological accuracy.
• Quality Control in Pharmaceutical Manufacturing: Used to monitor the purity and stability of nucleoside active pharmaceutical ingredients (APIs) and finished drug products.

Basic Information

Product Name	[5',5''-2H2]2'-Deoxyguanosine Monohydrate
CAS No.	478511-28-3
Molecular Formula	C10H12D2N5O4 • H2O
Molecular Weight	307.28 g/mol (anhydrous basis)
Synonyms	2'-Deoxyguanosine-5',5''-d2 Monohydrate; 2'-Deoxyguanosine-d2 (5',5''); 2-Amino-1,9-dihydro-9-[(2R,4S,5R)-4-hydroxy-5-(hydroxymethyl-d2)tetrahydro-2-furanyl]-6H-purin-6-one Monohydrate; dG-d2; Deuterated 2'-Deoxyguanosine; Guanosine, 2'-deoxy-, 5',5''-d2-, monohydrate
EINECS	Contact for details

Quality Control

Our [5',5''-2H2]2'-Deoxyguanosine Monohydrate is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including NMR and mass spectrometry for isotopic enrichment verification and HPLC for chemical and isomeric purity, to ensure it meets the exacting standards required for analytical applications. A comprehensive Certificate of Analysis (COA) detailing isotopic purity, chemical purity, and residual solvent levels is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C. This product is hygroscopic (moisture-sensitive). For long-term storage, desiccate at -20°C. Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (NMR)	Conforms to structure
Isotopic Purity (Deuterium Incorporation)	≥ 98 atom % D
Chemical Purity (HPLC)	≥ 98.0%
Water Content (Karl Fischer)	5.0 - 8.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.