Description

[5',5''-2H2]2'-Deoxycytidine Monohydrate is a stable isotope-labeled nucleoside derivative, specifically deuterated at the 5' and 5'' positions of the deoxyribose sugar. This high-purity compound is essential for use as an internal standard in quantitative mass spectrometry, ensuring accurate and reliable analytical results. It is primarily utilized by researchers and manufacturers in pharmaceutical development, clinical diagnostics, and advanced metabolomics studies where precise quantification of nucleoside analogs is critical.

Application

• Mass Spectrometry Internal Standard: Serves as a critical reference compound for the accurate quantification of 2'-deoxycytidine and related analogs in LC-MS/MS and GC-MS analyses.
• Pharmaceutical R&D: Used in the development and pharmacokinetic/pharmacodynamic (PK/PD) studies of antiviral and anticancer nucleoside drugs.
• Metabolomics Research: Enables precise tracking and measurement of deoxycytidine metabolism in cellular and biological systems.
• Clinical Diagnostics: Applied in assays for detecting abnormalities in nucleoside metabolism, which can be biomarkers for certain diseases.
• Isotope Dilution Analysis: A key component in high-accuracy methods for determining the concentration of natural nucleosides in complex matrices.
• Biochemical Tracing: Facilitates studies on DNA synthesis and repair mechanisms due to its incorporated stable deuterium labels.

Basic Information

Product Name	[5',5''-2H2]2'-Deoxycytidine Monohydrate
CAS No.	478511-25-0
Molecular Formula	C9H112H2N3O4 • H2O
Molecular Weight	263.25 g/mol (for monohydrate)
Synonyms	2'-Deoxy-5,5-dideuteriocytidine Monohydrate; 1-(2-Deoxy-5,5-dideuterio-β-D-erythro-pentofuranosyl)-4-aminopyrimidin-2(1H)-one Monohydrate; dC-d2 Monohydrate; Deuterated Deoxycytidine; Cytosine Deoxyriboside-d2; 2'-Deoxycytidine-5,5-d2; Stable Isotope-Labeled 2'-Deoxycytidine
EINECS	Contact for details

Quality Control

Our [5',5''-2H2]2'-Deoxycytidine Monohydrate is manufactured under strict quality systems to ensure exceptional isotopic and chemical purity, critical for its role as an analytical standard. Each batch is supported by a comprehensive Certificate of Analysis (COA) detailing parameters such as isotopic enrichment (atom % D), chemical purity by HPLC, and confirmation of structure. We adhere to relevant guidelines for the production of stable isotope-labeled compounds for research use.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C. This product is hygroscopic (moisture-sensitive). For long-term storage, consider desiccation. Keep container tightly sealed in a dry and well-ventilated place.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Enrichment	≥ 98 atom % D
Water Content (KF)	5.0 - 8.0% (Theoretical for monohydrate: ~6.8%)
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.