Description

[5',5''-2H2]Cytidine CAS NO 478511-21-6 is a deuterium-labeled isotopologue of the essential nucleoside cytidine, where two hydrogen atoms at the 5' and 5'' positions are replaced by deuterium. This stable isotope labeling is critical for advanced analytical research, providing a non-radioactive tracer with distinct mass spectral properties. It is primarily utilized by researchers and manufacturers in the pharmaceutical, biotechnology, and analytical chemistry sectors for applications in drug metabolism studies (DMPK), quantitative mass spectrometry, and as an internal standard for precise bioanalytical measurements.

Application

• Internal Standard for LC-MS/MS: Serves as a crucial, high-purity internal standard for the accurate quantification of cytidine and its metabolites in complex biological matrices like plasma, urine, and tissue homogenates.
• Drug Metabolism and Pharmacokinetics (DMPK): Used as a stable isotope-labeled tracer to study the absorption, distribution, metabolism, and excretion (ADME) profiles of nucleoside-based pharmaceuticals.
• Biochemical Pathway Analysis: Enables the tracing and quantification of cytidine incorporation into RNA and related nucleotide pools within cellular systems.
• Deuterated Reference Material: Acts as a certified reference material (CRM) for calibrating analytical instruments and validating methods in metabolomics and proteomics research.
• Synthesis of Labeled Compounds: Used as a key building block for the synthesis of more complex deuterated molecules for research and diagnostic purposes.

Basic Information

Product Name	[5',5''-2H2]Cytidine
CAS No.	478511-21-6
Molecular Formula	C9H11D2N3O5
Molecular Weight	245.22 g/mol
Synonyms	1-((2R,3R,4S,5R)-3,4-Dihydroxy-5-((2H2)hydroxymethyl)tetrahydrofuran-2-yl)-4-amino-2(1H)-pyrimidinone; Cytidine-5',5''-d2; 5',5''-Dideuteriocytidine; Deuterated Cytidine; [5',5''-2H2]-Cytidine; Cytidine-d2; 4-Amino-1-[(2R,3R,4S,5R)-3,4-dihydroxy-5-[(2H2)hydroxymethyl]oxolan-2-yl]pyrimidin-2-one
EINECS	Contact for details

Quality Control

Our [5',5''-2H2]Cytidine is manufactured under strict quality systems. Each batch undergoes comprehensive analytical testing, including NMR (1H, 13C) for structural confirmation and deuterium incorporation, HPLC for chemical purity, and mass spectrometry for isotopic enrichment verification. We provide a detailed Certificate of Analysis (COA) with every shipment, ensuring traceability and compliance with your research-grade specifications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below for long-term stability. The product is hygroscopic; allow the vial to equilibrate to room temperature in a desiccator before opening to prevent moisture absorption.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (NMR)	Conforms to structure
Isotopic Purity (Deuterium Enrichment)	≥ 98 atom % D
Chemical Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.