Description

[5',5''-2H2]2'-Deoxyadenosine Monohydrate is a stable isotope-labeled nucleoside derivative, specifically deuterated at the 5' and 5'' positions of the deoxyribose sugar. This compound is essential as an internal standard in quantitative mass spectrometry-based assays, providing unmatched accuracy for pharmacokinetic and metabolic studies. It is primarily utilized by pharmaceutical research organizations, contract research laboratories, and academic institutions engaged in advanced drug development and biomarker discovery.

Application

• LC-MS/MS Internal Standard: Critical for the precise quantification of 2'-deoxyadenosine and related metabolites in biological matrices like plasma, urine, and tissue homogenates.
• Pharmacokinetic (PK) and Bioanalytical Studies: Enables accurate tracking of drug absorption, distribution, metabolism, and excretion (ADME) for nucleoside-based therapeutics.
• Metabolomics Research: Serves as a stable isotope tracer to elucidate metabolic pathways and flux analysis in cellular systems.
• Diagnostic Assay Development: Used in the calibration and validation of clinical diagnostic tests for genetic disorders and cancer biomarkers linked to nucleotide metabolism.
• Quality Control for Pharmaceutical Manufacturing: Acts as a reference material to ensure the purity and consistency of nucleoside active pharmaceutical ingredients (APIs).
• Biochemical Research: Employed in enzymatic studies to investigate the mechanisms of DNA polymerases and other nucleotide-processing enzymes.

Basic Information

Product Name	[5',5''-2H2]2'-Deoxyadenosine Monohydrate
CAS No.	478510-81-5
Molecular Formula	C10H12D2N5O4 · H2O
Molecular Weight	285.28 g/mol (anhydrous basis: 267.25)
Synonyms	2'-Deoxyadenosine-5',5''-d2 Monohydrate; 9-[(2R,5S)-5-(Hydroxymethyl-d2)tetrahydrofuran-2-yl]-9H-purin-6-amine Monohydrate; dA-d2; Deuterated 2'-Deoxyadenosine; 2'-Deoxyadenosine-d2; Adenine Deoxyriboside-d2; Stable Isotope Labeled dA; 2'-dAdo-d2
EINECS	Contact for details

Quality Control

Every batch of [5',5''-2H2]2'-Deoxyadenosine Monohydrate is manufactured and analyzed under strict quality management systems. We provide comprehensive analytical data to ensure the product meets the high standards required for sensitive mass spectrometry applications. A detailed Certificate of Analysis (COA) is supplied with each shipment, reporting key parameters including isotopic purity (deuterium enrichment), chemical purity by HPLC, and identity confirmation by NMR and MS.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. This material is hygroscopic (moisture-sensitive) and light-sensitive. For long-term storage, consider desiccation and storage under inert atmosphere to maintain optimal stability and isotopic integrity.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Purity (Deuterium Enrichment)	≥ 98 atom % D
Water Content (KF)	5.0 - 8.0% (Theoretical for monohydrate: ~6.3%)
Residue on Ignition	≤ 0.1%
Heavy Metals	≤ 10 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.