Description

1-Hydroxy-4-[(Hydroxyimino)Methyl]-2,2,5,5-Tetramethyl-2,5-Dihydro-1H-Imidazol-3-Ium-3-Olate is a specialized nitroxide radical compound, also known as a hydroxyimino-substituted imidazoline nitroxide. This compound serves as a highly effective and stable spin label and spin trap in advanced research applications, enabling the study of molecular dynamics and free radical processes. It is primarily utilized by researchers and product developers in the pharmaceutical, biotech, and materials science sectors for applications ranging from protein structure analysis to polymer characterization.

Application

• Spin Labeling in Biophysics: Used to tag proteins, nucleic acids, and membranes for Electron Paramagnetic Resonance (EPR) spectroscopy to study structure, dynamics, and conformational changes.
• Free Radical Research: Acts as a spin trap to detect, identify, and quantify short-lived free radicals in chemical and biological systems.
• Polymer Science: Employed as a probe to investigate polymer chain mobility, cross-linking density, and degradation mechanisms via EPR.
• Antioxidant Studies: Utilized in model systems to study the mechanisms of antioxidant activity and oxidative stress.
• Catalysis Research: Serves as a paramagnetic probe to monitor reaction intermediates and catalytic cycles in homogeneous and heterogeneous catalysis.
• Diagnostic Agent Development: Investigated for potential use in functional MRI (fMRI) contrast agents and other diagnostic imaging techniques.

Basic Information

Product Name	1-Hydroxy-4-[(Hydroxyimino)Methyl]-2,2,5,5-Tetramethyl-2,5-Dihydro-1H-Imidazol-3-Ium-3-Olate
CAS No.	49837-80-1
Molecular Formula	C9H16N3O3+•O-
Molecular Weight	214.24 g/mol
Synonyms	4-Hydroxyiminomethyl-2,2,5,5-tetramethyl-δ³-imidazoline-3-oxide-1-oxyl; 4-(Hydroxyiminomethyl)-2,2,5,5-tetramethyl-3-imidazoline-1-oxyl-3-oxide; 4-Formyl-2,2,5,5-tetramethyl-3-imidazoline-1-oxyl-3-oxime; 4-Carboximidoyl-2,2,5,5-tetramethyl-3-imidazoline-1-oxyl-3-oxide; Hydroxyimino-methyl-TEMPO; HIM-TEMPO; Nitroxide spin label; Stable free radical.
EINECS	Contact for details

Quality Control

Our production of this high-purity nitroxide radical adheres to stringent quality protocols. Every batch is characterized using advanced analytical techniques including HPLC, EPR spectroscopy, and NMR to confirm identity, purity, and radical concentration. Certificates of Analysis (COA) detailing assay, impurity profile, and specific tests are provided with each shipment to ensure traceability and compliance with research-grade specifications.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive); ensure the container is sealed under an inert atmosphere if specified and stored in a low-humidity environment to prevent degradation.

Specification

Item	Specification
Appearance	Orange to red crystalline powder
Identification (IR)	Conforms to structure
Identification (EPR)	Characteristic nitroxide radical signal
Assay (HPLC)	≥ 97.0%
Radical Content (EPR)	≥ 95.0%
Loss on Drying	≤ 2.0%
Residue on Ignition	≤ 0.5%
Heavy Metals (as Pb)	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.