Description

Phenolphthalein Phosphate Disodium Salt is a key chromogenic substrate used in enzymatic assays, particularly for the detection of alkaline phosphatase activity. Its value lies in its ability to produce a distinct, measurable color change upon enzymatic hydrolysis, enabling sensitive and quantitative analysis. This compound is essential for researchers and quality control laboratories in the pharmaceutical, diagnostic, and life science research sectors, where precise enzyme detection is critical.

Application

• Enzymatic Assay Substrate: Primary substrate for the colorimetric detection and quantification of alkaline phosphatase (ALP) activity in biochemical and clinical diagnostics.
• Diagnostic Kits: A critical component in commercial diagnostic test kits and strips for medical and veterinary applications.
• Life Science Research: Used in ELISA (Enzyme-Linked Immunosorbent Assay), Western blotting, and other immunoassay techniques for signal generation.
• Pharmaceutical Quality Control: Employed in testing procedures to monitor enzyme activity and ensure batch consistency in biopharmaceutical production.
• Histochemistry & Cell Staining: Utilized as a substrate in histological staining to localize alkaline phosphatase activity in tissue samples.
• Food & Environmental Testing: Applied in assays designed to detect bacterial contamination or specific enzymatic markers in food safety and environmental monitoring.

Basic Information

Product Name	Phenolphthalein Phosphate Disodium Salt
CAS No.	41452-31-7
Molecular Formula	C20H14O8PNa2
Molecular Weight	468.26 g/mol
Synonyms	Phenolphthalein Diphosphate Disodium Salt; Phenolphthalein Phosphate, Disodium Salt; Disodium Phenolphthalein Phosphate; ALP Substrate; Phosphatase Substrate; 3,3-Bis(4-hydroxyphenyl)-1(3H)-isobenzofuranone 4,4'-bis(dihydrogen phosphate) disodium salt; NSC 401443; Phenolphthalein bis(dihydrogen phosphate) disodium salt
EINECS	Contact for details

Quality Control

Our Phenolphthalein Phosphate Disodium Salt is manufactured under strict quality systems to ensure high purity and consistent performance for critical diagnostic and research applications. Each batch is supported by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We adhere to relevant industry standards and our quality commitment ensures reliable and reproducible results in your enzymatic assays.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive); ensure the container is kept tightly sealed in a low-humidity environment to prevent degradation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Water Content	≤ 2.0%
Solubility	Clear, colorless solution in water
Enzymatic Activity	Suitable for use as ALP substrate

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.