Description

Cytidine-5,6-D2 is a stable isotope-labeled analog of the essential nucleoside cytidine, specifically deuterated at the 5 and 6 positions of the pyrimidine ring. This precise labeling is critical for applications requiring high sensitivity and minimal background interference in analytical techniques. It is primarily utilized by researchers and manufacturers in the pharmaceutical, biotechnology, and analytical chemistry sectors for advanced metabolic studies, drug development, and as an internal standard for quantitative mass spectrometry.

Application

• Internal Standard for Quantitative Analysis: Used in LC-MS and GC-MS for the accurate quantification of cytidine and related metabolites in complex biological matrices.
• Metabolic Pathway Tracing: Enables detailed investigation of nucleoside and nucleotide metabolism, RNA synthesis, and pharmacokinetic studies in drug discovery.
• Pharmaceutical Research: Serves as a key reagent in the development and testing of antiviral and anticancer nucleoside analogs.
• Biochemical Assays: Employed in enzymatic studies to monitor reactions involving cytidine deaminase and other nucleoside-modifying enzymes.
• NMR Spectroscopy: Acts as a valuable tool for structural elucidation and dynamic studies due to the distinct properties of deuterium.
• Stable Isotope Labeling: Essential for producing labeled biomolecules for advanced proteomics and metabolomics research.

Basic Information

Product Name	Cytidine-5,6-D2
CAS No.	40632-25-5
Molecular Formula	C9H11D2N3O5
Molecular Weight	245.22 g/mol
Synonyms	1-β-D-Ribofuranosyl-5,6-dideuteriocytosine; 4-Amino-5,6-dideuterio-1-β-D-ribofuranosyl-2(1H)-pyrimidinone; Cytidine-5,6-d2; [5,6-D2]Cytidine; Deuterated Cytidine; Cytidine-d2; Stable Isotope-Labeled Cytidine
EINECS	Contact for details

Quality Control

Our Cytidine-5,6-D2 is manufactured under strict quality systems to ensure batch-to-batch consistency and high isotopic purity. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing parameters such as chemical purity, isotopic enrichment, and absence of impurities. We support research and development with materials suitable for use under cGMP conditions where applicable.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥98.0%
Isotopic Enrichment	≥98 atom % D
Water Content (KF)	≤1.0%
Residue on Ignition	≤0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.