Description

Uridine-5,6-D2 is a deuterium-labeled isotopologue of the essential nucleoside uridine, specifically deuterated at the 5 and 6 positions of the pyrimidine ring. This stable isotope-labeled compound is critical for advanced analytical and metabolic research, providing a non-radioactive tracer with distinct mass spectral properties. It is primarily utilized by researchers and manufacturers in the pharmaceutical, biotechnology, and diagnostic sectors for applications requiring precise tracking and quantification.

Application

• Internal Standard for Mass Spectrometry (MS): Serves as a critical reference standard in LC-MS and GC-MS analyses for the accurate quantification of endogenous uridine and related metabolites in complex biological matrices.
• Metabolic Pathway Tracing: Used in stable isotope labeling studies (SILS) and flux analysis to investigate nucleotide metabolism, RNA synthesis, and cellular energy pathways.
• Pharmaceutical Research & Development: Employed in drug metabolism and pharmacokinetics (DMPK) studies to trace the metabolic fate of nucleoside-based drug candidates.
• Biochemical Assay Development: Acts as a key component in developing diagnostic assays and enzymatic activity tests that require differentiation from naturally occurring uridine.
• NMR Spectroscopy: Useful as a probe in nuclear magnetic resonance studies due to the distinct properties of deuterium, aiding in structural and dynamic analysis of biomolecules.
• Synthesis of Labeled Biomolecules: Functions as a building block for the synthesis of more complex deuterium-labeled nucleotides, oligonucleotides, or RNA strands.

Basic Information

Product Name	Uridine-5,6-D2
CAS No.	40632-21-1
Molecular Formula	C9H10D2N2O6
Molecular Weight	246.21 g/mol
Synonyms	1-((2R,3R,4S,5R)-3,4-Dihydroxy-5-(hydroxymethyl)oxolan-2-yl)-5,6-dideuteriopyrimidine-2,4-dione; 5,6-Dideuterouridine; [5,6-D2]Uridine; Uridine-d2; Deuterated Uridine; Uridine (5,6-D2); 2,4(1H,3H)-Pyrimidinedione, 1-β-D-ribofuranosyl-, 5,6-d2-
EINECS	Contact for details

Quality Control

Our Uridine-5,6-D2 is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing to ensure high chemical purity, isotopic enrichment, and batch-to-batch consistency. A comprehensive Certificate of Analysis (COA) detailing purity (by HPLC), isotopic purity (by MS), and identity confirmation is provided with every shipment. We support research and production needs compliant with cGMP and ISO standards where applicable.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature (15-25°C) in a dry environment. For long-term storage, consider storing at -20°C under inert atmosphere to ensure maximum stability.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Purity (D2)	≥ 98 atom % D
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.