Description

2-Iminothiolane is a versatile heterocyclic organic compound, also known as Traut's Reagent, widely recognized for its role as a thiolation reagent. Its primary value lies in its ability to introduce sulfhydryl groups into proteins, peptides, and other molecules under mild aqueous conditions, facilitating conjugation, labeling, and structural studies. This product is essential for researchers and manufacturers in the biotechnology, pharmaceutical development, and diagnostic kit production sectors, where precise biomolecule modification is critical.

Application

• Protein and Antibody Modification: Introduction of thiol groups for site-specific conjugation with maleimide-activated dyes, drugs, or surfaces.
• Bioconjugation and Cross-linking: A key reagent for creating stable thioether linkages in the synthesis of antibody-drug conjugates (ADCs) and other bioconjugates.
• Peptide Engineering: Used to modify cysteine residues or introduce new thiol handles into synthetic peptides for structural and functional studies.
• Immobilization Chemistry: Functionalization of gold surfaces, nanoparticles, or chromatography resins with biomolecules via thiol-gold or disulfide bonds.
• Diagnostic Reagent Synthesis: Production of enzyme-labeled antibodies and other reagents used in ELISA, lateral flow assays, and other diagnostic platforms.
• Polymer Functionalization: Thiolation of polymers and dendrimers to create reactive scaffolds for further chemical modification.

Basic Information

Product Name	2-Iminothiolane
CAS No.	4781-83-3
Molecular Formula	C4H8ClNS
Molecular Weight	137.63 g/mol
Synonyms	Traut's Reagent; 2-Iminothiolane hydrochloride; 2-IT; 4-Thiobutyrimidate hydrochloride; 2-Iminotetrahydrothiophene hydrochloride; 1-Aza-4-thiacyclohexane hydrochloride; 2-Immonium-1,3-thiolane chloride; (Tetrahydro-2H-thiopyran-2-ylidene)amine hydrochloride
EINECS	225-320-2

Quality Control

Our 2-Iminothiolane is produced under strict quality management systems to ensure batch-to-batch consistency and high purity, suitable for sensitive bioconjugation applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We support compliance with cGMP and ISO 9001 standards for pharmaceutical and research use, ensuring reliable supply chain integrity.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability and reactivity. Allow the sealed container to reach room temperature before opening to minimize moisture ingress.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Solubility	Clear, colorless solution in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.