Description

2-Methoxy-17Beta-Estradiol-1,4,16,16,17-D5 is a deuterium-labeled analog of 2-methoxyestradiol, a key metabolite of estradiol with significant research interest. This stable isotope-labeled compound is essential for use as an internal standard in mass spectrometry-based analytical methods, ensuring precise quantification and method validation. It is primarily utilized by pharmaceutical R&D laboratories, contract research organizations (CROs), and academic institutions engaged in steroid hormone metabolism studies, pharmacokinetic research, and bioanalytical assay development.

Application

• Internal Standard for LC-MS/MS: Critical for the accurate quantification of 2-methoxyestradiol and related metabolites in complex biological matrices like plasma, serum, and tissue homogenates.
• Drug Metabolism and Pharmacokinetics (DMPK): Used in tracer studies to investigate the metabolic pathways, bioavailability, and clearance rates of estradiol derivatives.
• Biomarker Research: Enables the development of sensitive assays for endogenous steroid hormones in clinical and preclinical research settings.
• Pharmaceutical Quality Control: Supports the analysis of drug substances and products containing related steroid compounds for purity and impurity profiling.
• Endocrinology Studies: A vital tool for research into estrogen signaling, cancer biology (particularly anti-angiogenic effects), and other hormone-mediated processes.
• Certified Reference Material (CRM): Suitable for use in the preparation of certified reference materials for laboratory accreditation and method standardization.

Basic Information

Product Name	2-Methoxy-17Beta-Estradiol-1,4,16,16,17-D5
CAS No.	358731-34-7
Molecular Formula	C19H21D5O3
Molecular Weight	305.43 g/mol
Synonyms	2-Methoxyestra-1,3,5(10)-triene-3,17β-diol-1,4,16,16,17-D5; 2-MeOE2-d5; Deuterated 2-Methoxyestradiol; 2-Methoxy-17β-estradiol-d5; 2-Methoxyestra-1,3,5(10)-triene-3,17β-diol (1,4,16,16,17-D5); 2-Methoxy-17β-oestradiol-1,4,16,16,17-D5; 2-Methoxy-E2-d5
EINECS	Contact for details

Quality Control

Every batch of 2-Methoxy-17Beta-Estradiol-1,4,16,16,17-D5 is produced and analyzed under strict quality management systems. We guarantee high chemical and isotopic purity to meet the exacting demands of analytical research. Comprehensive testing includes identity confirmation, assay by HPLC, and determination of deuterium enrichment by NMR or MS. A detailed Certificate of Analysis (COA) providing lot-specific data is supplied with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere for long-term stability. This compound is hygroscopic (moisture-sensitive). Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Purity (D-enrichment)	≥ 98 atom % D
Chemical Purity (by HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.