Description

2-Ethyl-D5-2-Hydroxybutyric-3,3,4,4,4-D5 Acid CAS NO 358731-27-8 is a high-purity, stable isotope-labeled derivative of 2-ethyl-2-hydroxybutyric acid, where five hydrogen atoms are replaced by deuterium. This compound is essential for advanced analytical research, providing a critical internal standard for precise quantification and metabolic pathway tracing in complex biological matrices. It is primarily required by pharmaceutical R&D laboratories, contract research organizations (CROs), and academic institutions engaged in pharmacokinetic studies, drug metabolism research (DMPK), and biomarker discovery using techniques like LC-MS and GC-MS.

Application

• Internal Standard for Mass Spectrometry: Serves as a critical quantitative reference in Liquid Chromatography-Mass Spectrometry (LC-MS) and Gas Chromatography-Mass Spectrometry (GC-MS) analysis of its unlabeled counterpart or related metabolites.
• Drug Metabolism and Pharmacokinetics (DMPK): Used in tracing and quantifying the absorption, distribution, metabolism, and excretion (ADME) of pharmaceutical compounds in preclinical and clinical studies.
• Metabolomics and Biomarker Research: Enables accurate profiling of endogenous metabolites in biological fluids (plasma, urine) for disease biomarker discovery and metabolic pathway analysis.
• Stable Isotope Labeling Studies: Facilitates research into biochemical mechanisms and reaction kinetics where tracking specific atoms is necessary.
• Pharmaceutical Impurity Profiling: Aids in the identification and quantification of process-related impurities or degradation products during drug development and quality control.
• Forensic and Toxicology Analysis: Provides a reliable standard for the confirmatory analysis of specific compounds in forensic toxicology screens.

Basic Information

Product Name	2-Ethyl-D5-2-Hydroxybutyric-3,3,4,4,4-D5 Acid
CAS No.	358731-27-8
Molecular Formula	C6H5D5O3
Molecular Weight	~139.17 g/mol (exact mass varies with isotopic distribution)
Synonyms	2-Ethyl-2-hydroxybutyric-3,3,4,4,4-d5 acid; (3,3,4,4,4-Pentadeutero-2-ethyl-2-hydroxybutanoic acid); Deuterated 2-Ethyl-2-hydroxybutyric Acid; 2-Ethyl-2-hydroxy[3,3,4,4,4-2H5]butanoic Acid; Ethylhydroxybutyric Acid-d5; 2-Ethyl-2-hydroxybutyric Acid (D5, 98%); Stable Isotope Labeled 2-EHA; Internal Standard for 2-Ethyl-2-hydroxybutyrate
EINECS	Contact for details

Quality Control

Every batch of our 2-Ethyl-D5-2-Hydroxybutyric Acid is manufactured and analyzed under strict quality management protocols to ensure exceptional isotopic and chemical purity, critical for sensitive analytical applications. We provide comprehensive Certificates of Analysis (COA) detailing isotopic enrichment (D-content), chemical purity by HPLC, and identity confirmation by NMR and MS. Our quality systems support compliance with the rigorous standards required for research and development in regulated environments.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a cool, dry place. This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to minimize exposure to atmospheric humidity. For long-term storage, consider storing under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥98.0%
Isotopic Enrichment	≥98 atom % D
Chemical Purity (by titration)	≥97.0%
Water Content (KF)	≤1.0%
Residue on Ignition	≤0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.