Description

Piperazine-N,N'-Bis(2-Ethanesulfonic Acid)-D18 is a deuterated analog of the widely used biological buffer PIPES, specifically designed for applications requiring isotopic labeling. This compound is essential for providing a stable, non-interacting buffering environment in sensitive analytical techniques. It is primarily utilized by researchers and manufacturers in the fields of nuclear magnetic resonance (NMR) spectroscopy, mass spectrometry, and structural biology where deuterium substitution is critical for reducing background signal and enhancing resolution.

Application

• Internal standard or reference material for quantitative NMR spectroscopy and LC-MS/MS analysis.
• Deuterated buffer component for structural biology studies, including protein crystallography and solution-state NMR of biomolecules.
• Use in metabolic research and isotope tracing experiments to study biochemical pathways.
• Critical reagent in the development and validation of analytical methods for pharmaceutical quality control.
• Component in specialized formulations for diagnostic assay kits requiring high-purity, isotopically labeled chemicals.
• Research chemical for investigating enzyme kinetics and biophysical properties under controlled, deuterated conditions.

Basic Information

Product Name	Piperazine-N,N'-Bis(2-Ethanesulfonic Acid)-D18
CAS No.	352534-95-3
Molecular Formula	C8D18N2O6S2
Molecular Weight	~330.5 g/mol
Synonyms	PIPES-d18; Deuterated PIPES; Piperazine-1,4-bis(2-ethanesulfonic acid)-d18; 1,4-Piperazinediethanesulfonic acid-d18; PIPES Buffer-d18; [2,2,2',2',3,3,3',3',-²H8; 1,1',4,4'-²H4]PIPES
EINECS	Contact for details

Quality Control

Our Piperazine-N,N'-Bis(2-Ethanesulfonic Acid)-D18 is produced under strict quality management systems. Each batch undergoes rigorous analytical testing to ensure high isotopic purity and chemical integrity, meeting the stringent requirements for research and analytical applications. Certificates of Analysis (COA) detailing specifications such as isotopic enrichment (D-atom %), chemical purity, and NMR conformity are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This material is hygroscopic (moisture-sensitive). For long-term storage, consider desiccation or storage under an inert atmosphere to maintain stability and isotopic purity.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Purity (D-atom %)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.