Description

Bis-Tris-D19 CAS NO 352534-93-1 is a deuterated analog of the widely used biological buffer, Bis-Tris, where nineteen hydrogen atoms are replaced with deuterium. This isotopic labeling is critical for applications requiring nuclear magnetic resonance (NMR) spectroscopy, as it reduces background signal interference and simplifies spectral analysis. It is an essential reagent for structural biologists, analytical chemists, and pharmaceutical researchers engaged in protein dynamics studies, metabolic pathway tracing, and advanced material science.

Application

• NMR Spectroscopy Solvent & Buffer: Used as a deuterated buffer component for studying biomolecular structures and dynamics in deuterium oxide (D₂O) solutions.
• Protein Crystallography: Serves as a crystallization agent and pH buffer for stabilizing protein structures in deuterated environments.
• Metabolic Research & Tracing: Employed in stable isotope labeling experiments to track metabolic fluxes and pathways in biological systems.
• Pharmaceutical Development: Utilized in the formulation and analysis of deuterated drug compounds and in quality control for NMR-based assays.
• Material Science Research: Acts as a structuring agent or pH moderator in the synthesis and analysis of deuterated polymers and advanced materials.
• Academic & Industrial R&D: A fundamental tool in research laboratories for any application requiring precise pH control in a deuterated matrix.

Basic Information

Product Name	Bis-Tris-D19
CAS No.	352534-93-1
Molecular Formula	C8D19NO5
Molecular Weight	213.30 g/mol (deuterated form)
Synonyms	2-[Bis(2-hydroxyethyl)amino]-2-(hydroxymethyl)-1,3-propanediol-d19; Bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane-d19; Bis-Tris-d19; BIS-TRIS (Deuterated); 1,3-Bis[tris(hydroxymethyl)methylamino]propane-d19 (related compound); BIS TRIS Deuterated; Deuterated BIS-TRIS Buffer; NMR Buffer-d19
EINECS	Contact for details

Quality Control

Our Bis-Tris-D19 is manufactured under strict quality protocols to ensure high isotopic purity and chemical integrity, essential for sensitive spectroscopic applications. Each batch is supported by a comprehensive Certificate of Analysis (COA) detailing parameters such as isotopic enrichment (D-atom %), chemical purity, and NMR suitability. We adhere to cGMP principles for research-grade chemicals, ensuring traceability, consistency, and reliability for your critical research and development projects.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This compound is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent absorption of atmospheric moisture, which can affect purity and isotopic integrity.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Deuterium Content (NMR)	≥ 98 atom % D
Purity (HPLC)	≥ 99.0%
pH (in D₂O)	5.5 - 6.5 (1M solution)
Water Content (KF)	≤ 0.5%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.