Description

5-(Hydroxymethyl-D2)Uracil-6-D1 is a deuterium-labeled derivative of uracil, a fundamental pyrimidine nucleobase. This isotopically labeled compound is critical for advanced research and development, providing a stable, non-radioactive tracer for mechanistic studies and analytical method development. It is primarily utilized by pharmaceutical researchers, analytical chemists, and isotope labeling specialists in drug metabolism and pharmacokinetics (DMPK) studies, proteomics, and as a reference standard in mass spectrometry.

Application

• Internal Standard for LC-MS/MS: Serves as a crucial stable isotope-labeled internal standard for the quantitative bioanalysis of uracil and its analogs, ensuring high accuracy and precision in pharmacokinetic studies.
• Drug Metabolism Research (DMPK): Used as a tracer to elucidate metabolic pathways, study the absorption, distribution, metabolism, and excretion (ADME) of nucleoside-based pharmaceuticals.
• Proteomics and Biomarker Discovery: Employed in stable isotope labeling by amino acids in cell culture (SILAC) and other quantitative proteomics techniques to study cellular processes.
• Mechanistic Biochemical Studies: Facilitates the investigation of enzyme mechanisms, particularly those involving thymidylate synthase and other enzymes in nucleotide synthesis and catabolism.
• Nucleic Acid Research: Used in studies exploring DNA/RNA structure, stability, and repair mechanisms involving modified nucleobases.
• Reference Material: Acts as a high-purity certified reference material (CRM) for calibrating analytical instruments and validating analytical methods in regulatory environments.

Basic Information

Item	Detail
Product Name	5-(Hydroxymethyl-D2)Uracil-6-D1
CAS No.	352438-73-4
Molecular Formula	C5H3D3N2O3
Molecular Weight	130.11 g/mol
Synonyms	5-(Hydroxymethyl-d2)uracil-6-d1; [6-D,5-(Hydroxymethyl-d2)]Uracil; Uracil-5-(hydroxymethyl-d2)-6-d1; Deuterated Hydroxymethyluracil; 5-(Hydroxymethyl-2H2)uracil-6-2H; Isotopically Labeled Uracil Derivative; 5-HMU-d3; Stable Isotope-Labeled Nucleobase
EINECS	Contact for details

Quality Control

Every batch of 5-(Hydroxymethyl-D2)Uracil-6-D1 is manufactured and analyzed under strict quality management systems. We guarantee high chemical and isotopic purity, with comprehensive testing including HPLC for chemical purity and MS/NMR for isotopic enrichment verification. Certificates of Analysis (COA) are provided with each shipment, detailing batch-specific results. Our quality commitment supports compliance needs for GLP and GMP research environments.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive) and should be handled in a dry environment to prevent degradation. For long-term storage, consider desiccation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Purity (MS)	≥ 98 atom % D
Isotopic Enrichment (NMR)	≥ 95% at specified positions
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.