Description

7Beta-Hydroxycholesterol-25,26,26,26,27,27,27-D7 is a high-purity, deuterium-labeled analog of 7β-hydroxycholesterol, a key oxysterol metabolite. This stable isotope-labeled compound is essential for use as an internal standard in mass spectrometry-based analytical methods, ensuring precise quantification and method validation. It is critically needed by research laboratories, pharmaceutical developers, and CROs (Contract Research Organizations) engaged in lipidomics, metabolic disorder studies, and drug development research.

Application

• Internal Standard for LC-MS/MS: Primary application for the accurate quantification of endogenous 7β-hydroxycholesterol in biological matrices (plasma, serum, tissues).
• Biomarker Research: Used in studies investigating oxidative stress, atherosclerosis, neurodegenerative diseases (e.g., Alzheimer's), and other conditions where oxysterols serve as biomarkers.
• Drug Metabolism & Pharmacokinetics (DMPK): Employed in tracing cholesterol oxidation pathways and evaluating the metabolic fate of pharmaceutical compounds.
• Method Development & Validation: Critical for developing robust, GLP-compliant analytical methods in clinical and pre-clinical research settings.
• Lipidomics and Metabolomics: A key reagent in targeted and untargeted profiling of sterol and oxysterol pathways.
• Quality Control in Diagnostics: Used in the manufacture and calibration of diagnostic assays and kits for cardiovascular and metabolic health.

Basic Information

Product Name	7Beta-Hydroxycholesterol-25,26,26,26,27,27,27-D7
CAS No.	349553-97-5
Molecular Formula	C₂₇H₃₉D₇O₂
Molecular Weight	~409.7 g/mol
Synonyms	(25,26,26,26,27,27,27-D7)-7β-Hydroxycholesterol; 7β-Hydroxy-5-cholesten-3β-ol-25,26,26,26,27,27,27-d7; Deuterated 7β-Hydroxycholesterol; 7β-Hydroxycholesterol-d7; 7β-OH-Cholesterol-d7; Cholest-5-ene-3β,7β-diol-25,26,26,26,27,27,27-d7; 5-Cholestene-3β,7β-diol-25,26,26,26,27,27,27-d7
EINECS	Contact for details

Quality Control

Our 7Beta-Hydroxycholesterol-25,26,26,26,27,27,27-D7 is manufactured under strict quality systems. Each batch undergoes rigorous analytical testing, including HPLC, NMR, and MS, to ensure high chemical and isotopic purity and identity confirmation. Certificates of Analysis (COA) detailing batch-specific results are provided to guarantee traceability and consistency for your critical research applications.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below under an inert atmosphere. The product is hygroscopic and should be handled in a dry environment to prevent moisture uptake. Allow the vial to equilibrate to room temperature before opening to minimize condensation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC-MS)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 95% (Area %)
Isotopic Purity (Deuterium Enrichment)	≥ 98 atom % D
Chemical Purity	≥ 90%
Solubility	Soluble in ethanol, DMSO, chloroform

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.