Description

7Alpha-Hydroxycholesterol-25,26,26,26,27,27,27-D7 is a high-purity, deuterium-labeled analog of 7α-hydroxycholesterol, a key intermediate oxysterol in bile acid biosynthesis. This stable isotope-labeled compound is essential for use as an internal standard in mass spectrometry-based analytical methods, ensuring precise quantification and method validation. It is primarily required by pharmaceutical R&D laboratories, clinical research organizations, and analytical service providers specializing in lipidomics, metabolic pathway studies, and biomarker discovery.

Application

• Internal Standard for LC-MS/MS: Critical for the accurate quantification of endogenous 7α-hydroxycholesterol and related oxysterols in biological matrices (plasma, serum, tissue).
• Metabolic Pathway Tracing: Used in stable isotope dilution assays to study cholesterol metabolism and bile acid synthesis pathways.
• Biomarker Research: Enables precise analysis of oxysterol levels in studies related to liver diseases, atherosclerosis, and neurodegenerative disorders.
• Pharmaceutical Development: Supports drug metabolism and pharmacokinetics (DMPK) studies for compounds targeting cholesterol-related pathways.
• Quality Control & Method Development: Serves as a reference material in analytical method development, validation, and routine quality control in clinical and research laboratories.

Basic Information

Product Name	7Alpha-Hydroxycholesterol-25,26,26,26,27,27,27-D7
CAS No.	349553-94-2
Molecular Formula	C₂₇H₃₉D₇O₂
Molecular Weight	~409.7 g/mol
Synonyms	(25,26,26,26,27,27,27-Heptadeutero)-7α-hydroxycholesterol; 7α-Hydroxy-25,26,26,26,27,27,27-heptadeuterocholesterol; 7α-Hydroxycholesterol-d7; 5-Cholesten-3β-ol-7α-ol-25,26,26,26,27,27,27-d7; Deuterated 7α-Hydroxycholesterol; 7α-HC-d7; Stable Isotope Labeled 7α-Hydroxycholesterol
EINECS	Contact for details

Quality Control

Our 7Alpha-Hydroxycholesterol-25,26,26,26,27,27,27-D7 is manufactured under strict quality systems to ensure the high chemical and isotopic purity required for sensitive analytical applications. Each batch is supported by a comprehensive Certificate of Analysis (COA) detailing parameters such as chemical purity (by HPLC), isotopic enrichment, and absence of critical impurities. We adhere to cGMP principles for manufacturing and provide traceable documentation to support regulatory filings.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below under an inert atmosphere. Allow the sealed vial to equilibrate to room temperature before opening to prevent moisture condensation. Store away from strong oxidizers.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (IR / NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Enrichment	≥ 98 atom % D
Chemical Purity (by HPLC)	≥ 95.0%
Solubility	Soluble in ethanol, DMSO, chloroform

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.