Description

4-(Iodoacetamidomethyl)Cyclohexanecarboxylic Acid-Nhs is a high-purity, heterobifunctional crosslinking reagent designed for advanced bioconjugation and protein modification workflows. This compound is critical for creating stable covalent bonds between biomolecules, enabling precise labeling and immobilization in complex assays. It is primarily utilized by researchers and manufacturers in the pharmaceutical, diagnostic, and life science sectors for developing targeted therapeutics, biosensors, and affinity purification systems.

Application

• Bioconjugation Reagent: For covalently linking proteins, peptides, or antibodies to surfaces, particles, or other biomolecules via amine and thiol groups.
• Proteomics Research: Used in activity-based protein profiling (ABPP) and chemoproteomics to label and study enzyme active sites.
• Diagnostic Kit Development: Essential for immobilizing capture antibodies or antigens on solid supports in ELISA, lateral flow assays, and microarray platforms.
• Antibody-Drug Conjugate (ADC) Synthesis: Serves as a key linker precursor for constructing stable, site-specific conjugates between cytotoxic drugs and monoclonal antibodies.
• Affinity Chromatography Ligand Coupling: Enables the stable attachment of ligands to resin matrices for purifying specific proteins or compounds.
• Surface Functionalization: Modifies gold nanoparticles, quantum dots, or other nanomaterials for biomedical imaging and targeted delivery applications.
• Chemical Biology Tool: Facilitates the study of protein-protein interactions and cellular pathways through selective labeling.

Basic Information

Product Name	4-(Iodoacetamidomethyl)Cyclohexanecarboxylic Acid-Nhs
CAS No.	343803-27-0
Molecular Formula	C16H23IN2O5
Molecular Weight	450.27 g/mol
Synonyms	4-[(Iodoacetyl)amino]methylcyclohexanecarboxylic Acid N-Hydroxysuccinimide Ester; 4-(Iodoacetamidomethyl)Cyclohexane-1-carboxylic Acid NHS Ester; 4-(Iodoacetamidomethyl)Cyclohexanecarboxylic Acid Succinimidyl Ester; 4-(Iodoacetamidomethyl)Cyclohexanecarboxylic Acid N-Succinimidyl Ester; 4-(Iodoacetamidomethyl)Cyclohexanecarboxylic Acid, 2,5-Dioxopyrrolidin-1-yl Ester; 4-(Iodoacetamidomethyl)Cyclohexanecarboxylic Acid, N-Hydroxysuccinimide Ester; 4-(Iodoacetamidomethyl)Cyclohexanecarboxylic Acid, Active Ester; Iodoacetamidomethyl-CHC-NHS
EINECS	Contact for details

Quality Control

Our 4-(Iodoacetamidomethyl)Cyclohexanecarboxylic Acid-Nhs is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation via NMR and mass spectrometry, to ensure it meets the high standards required for sensitive research and development applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This material is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture uptake. For long-term storage, keep under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 95.0%
Assay	≥ 97.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMF, DMSO, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.