Description

Ethyl 4-Aminobenzoate-2,3,5,6-D4 is a deuterated analog of the common local anesthetic benzocaine, specifically labeled at the 2, 3, 5, and 6 positions of the aromatic ring. This high-purity stable isotope-labeled compound is essential for use as an internal standard in quantitative mass spectrometry, ensuring unparalleled accuracy and reproducibility in analytical methods. It is critically needed by pharmaceutical R&D laboratories, contract research organizations (CROs), and analytical testing facilities for drug metabolism and pharmacokinetics (DMPK) studies, bioanalytical method development, and forensic analysis.

Application

• Internal Standard for LC-MS/MS: Primary application as a quantitative reference standard in liquid chromatography-tandem mass spectrometry for the analysis of benzocaine and related compounds in biological matrices.
• Pharmaceutical Research: Used in drug metabolism studies (DMPK) to track the absorption, distribution, metabolism, and excretion of anesthetic and analgesic agents.
• Bioanalytical Method Development: Critical for developing and validating robust, high-sensitivity analytical methods in compliance with FDA and ICH guidelines.
• Forensic Toxicology: Employed in the precise quantification of benzocaine in forensic samples for legal and investigative purposes.
• Isotope Dilution Mass Spectrometry (IDMS): Serves as the ideal spike for IDMS, the gold-standard technique for achieving highest accuracy in concentration measurements.
• Metabolite Identification: Aids in the identification and quantification of metabolite products in complex biological systems.

Basic Information

Item	Detail
Product Name	Ethyl 4-Aminobenzoate-2,3,5,6-D4
CAS No.	342611-08-9
Molecular Formula	C9H7D4NO2
Molecular Weight	169.21 g/mol
Synonyms	Benzocaine-2,3,5,6-d4; Ethyl 4-Aminobenzoate-d4; 4-Aminobenzoic Acid Ethyl Ester-d4; p-Aminobenzoic Acid Ethyl Ester-d4; Anaesthesin-d4; Ethyl p-Aminobenzoate-d4; Benzocaine (phenyl-d4); Ethoforme-d4
EINECS	Contact for details

Quality Control

Every batch of Ethyl 4-Aminobenzoate-2,3,5,6-D4 is manufactured and analyzed to meet the stringent requirements for stable isotope-labeled internal standards. Our quality assurance includes rigorous testing for isotopic purity (D-enrichment), chemical purity, and absence of interferents. A comprehensive Certificate of Analysis (COA) is provided with each shipment, detailing results from NMR, HPLC, and MS analysis to ensure traceability and compliance with your method validation protocols.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation from atmospheric moisture.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (MS)	Conforms to structure
Purity (HPLC)	≥98.0% (Chemical Purity)
Isotopic Purity (D-enrichment)	≥98 atom % D
Water Content (KF)	≤0.5%
Residue on Ignition	≤0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.