Description

Fluorexon Trisodium Salt, Indicator Grade is a high-purity, water-soluble fluorescent chelating agent specifically formulated for analytical applications. This compound is critically important for the sensitive and selective detection of metal ions, particularly calcium, in complex matrices. It is an essential reagent for researchers and quality control laboratories in the pharmaceutical, biotechnology, and environmental analysis sectors who require reliable and precise indicators for titration and assay development.

Application

• Calcium Ion Detection: Primary use as a fluorescent indicator in complexometric titrations for calcium determination.
• Biochemical Assays: Key component in fluorometric assays for measuring intracellular calcium flux in cell biology and physiology research.
• Clinical Diagnostics: Used in the formulation of reagents for clinical analyzers to measure serum or plasma calcium levels.
• Water Quality Analysis: Employed in environmental testing kits and methods for determining water hardness (calcium and magnesium content).
• Pharmaceutical QC: Applied in the purity testing and assay development of calcium-containing active pharmaceutical ingredients (APIs) and formulations.
• Food & Beverage Analysis: Utilized for calcium fortification verification and nutritional labeling in dairy products, supplements, and beverages.

Basic Information

Product Name	Fluorexon Trisodium Salt, Indicator Grade
CAS No.	336616-50-3
Molecular Formula	C30H21NNa3O13
Molecular Weight	671.46 g/mol
Synonyms	Calcein, Fluorescein Complexon, Fluorescein-1,2-bis(o-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid trisodium salt; Fluorexon; NSC 113233; Calcein Sodium Salt; 2,7-Bis[bis(carboxymethyl)aminomethyl]fluorescein trisodium salt; Fluorescein-bis(methyliminodiacetic acid) trisodium salt
EINECS	Contact for details

Quality Control

Our Indicator Grade Fluorexon Trisodium Salt is manufactured under a quality management system and undergoes rigorous analytical testing to ensure batch-to-batch consistency and performance suitability for sensitive analytical applications. Each lot is supplied with a comprehensive Certificate of Analysis (COA) detailing purity, identity, and performance characteristics. We support compliance with relevant pharmacopeial and industry standards.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This material is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent absorption of atmospheric moisture, which may affect performance.

Specification

Item	Specification
Appearance	Orange-red to brownish-red powder
Identification (IR)	Conforms to reference spectrum
Assay (HPLC)	≥ 95.0% (on anhydrous basis)
Water Content (KF)	≤ 5.0 %
Fluorescence Performance	Conforms to reference standard
pH (1% Solution)	7.0 - 9.0

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.