Description

5-(Hydroxymethyl-D2)Uracil-6-D1 is a selectively deuterated derivative of uracil, a fundamental pyrimidine nucleobase. This compound is specifically designed for use as a stable isotope-labeled internal standard in advanced analytical chemistry, providing superior accuracy for quantitative mass spectrometry. It is an essential tool for researchers and quality control laboratories in the pharmaceutical, biotechnology, and diagnostic sectors, particularly for the precise quantification of nucleic acid metabolites, drug metabolites, and biomarkers.

Application

• Mass Spectrometry Internal Standard: Primary use as a stable isotope-labeled reference compound for the accurate quantification of uracil, 5-hydroxymethyluracil, and related metabolites via LC-MS/MS and GC-MS.
• Pharmaceutical Research & Development: Critical for pharmacokinetic (PK) and pharmacodynamic (PD) studies, enabling precise measurement of drug metabolism and nucleic acid analog therapeutics.
• Biomarker Analysis: Used in clinical and forensic toxicology to quantify modified nucleosides, which can serve as biomarkers for oxidative stress, cancer, and other diseases.
• Metabolomics & Proteomics: Facilitates high-precision quantitative analysis in systems biology studies investigating cellular metabolic pathways.
• Nucleic Acid Chemistry Research: Serves as a building block or probe in studies exploring the structure, function, and epigenetic modification of DNA and RNA.
• Quality Control & Method Validation: Employed by analytical laboratories to validate and ensure the accuracy and reproducibility of chromatographic methods for nucleobase analysis.

Basic Information

Product Name	5-(Hydroxymethyl-D2)Uracil-6-D1
CAS No.	334473-41-5
Molecular Formula	C5H3D3N2O3
Molecular Weight	130.11 g/mol (for monoisotopic mass)
Synonyms	5-(Hydroxymethyl-d2)uracil-6-d1; [D3]-5-Hydroxymethyluracil; 5-(Hydroxymethyl-1,2-d2)uracil-6-d; Isotopically Labeled 5-Hydroxymethyluracil; 5-Hydroxymethyluracil-d3; 5-Hydroxymethyl-2,4-dioxo-3,4-dihydropyrimidine-d3; HMU-d3; Stable Isotope Standard of 5hmU
EINECS	Contact for details

Quality Control

Every batch of 5-(Hydroxymethyl-D2)Uracil-6-D1 is manufactured and analyzed under strict quality management protocols to ensure exceptional isotopic purity and chemical integrity. Our products undergo rigorous quality testing to ensure compliance with industry standards. Certificates of Analysis (COA) are available upon request and provide detailed results for isotopic enrichment, chemical purity, and identity confirmation.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature (15-25°C) in a dry environment. For long-term storage, consider storing at 2-8°C to ensure maximum stability.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Enrichment	≥ 98 atom % D
Water Content (KF)	≤ 0.5%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.