Description

Dmt-Dg(Dmf) Phosphoramidite is a specialized, high-purity phosphoramidite monomer, a critical building block in automated oligonucleotide synthesis. Its primary value lies in enabling the precise and efficient incorporation of modified nucleosides into DNA and RNA sequences, which is essential for advanced research and therapeutic development. This compound is indispensable for scientists and manufacturers in the fields of antisense therapeutics, diagnostic probe development, and synthetic biology who require reliable, high-quality reagents for cutting-edge nucleic acid applications.

Application

• Oligonucleotide Synthesis: Key monomer for the solid-phase synthesis of DNA and RNA strands containing modified bases.
• Antisense & siRNA Therapeutics: Production of research-grade and therapeutic oligonucleotides designed for gene silencing and targeted therapies.
• Diagnostic Probes: Manufacture of labeled probes for PCR, FISH, and microarray technologies requiring specific sequence modifications.
• Synthetic Biology: Construction of novel genetic circuits and non-natural nucleic acid polymers (XNA).
• Academic & Industrial R&D: Fundamental research in biochemistry, molecular biology, and medicinal chemistry exploring nucleic acid function and drug discovery.
• API Intermediate: Serves as a critical raw material in the production chain for active pharmaceutical ingredients (APIs) based on oligonucleotides.

Basic Information

Product Name	Dmt-Dg(Dmf) Phosphoramidite
CAS No.	330628-04-1
Molecular Formula	C41H49N6O8P
Molecular Weight	784.85 g/mol
Synonyms	5'-O-(4,4'-Dimethoxytrityl)-2'-deoxyguanosine, N2-(Dimethylaminomethylene)-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite; DMT-dG(DMF)-CE Phosphoramidite; N2-DMF-dG Phosphoramidite; 2'-Deoxyguanosine, 5'-O-[bis(4-methoxyphenyl)phenylmethyl]-2-[[(dimethylamino)methylene]amino]-N-[2-[(1,1-dimethylethyl)dimethylsilyl]oxy]ethyl]-N-[2-[(2-cyanoethoxy)(diisopropylamino)phosphino]oxy]ethyl]-, (R)-; Guanosine Phosphoramidite, DMT-protected.
EINECS	Contact for details

Quality Control

Every batch of Dmt-Dg(Dmf) Phosphoramidite is manufactured and tested under strict quality systems to ensure it meets the exacting standards required for sensitive oligonucleotide synthesis. Our quality assurance includes rigorous analytical testing by HPLC, NMR, and 31P NMR to confirm identity, purity, and reactivity. A comprehensive Certificate of Analysis (COA) detailing all specifications is provided with each shipment to guarantee traceability and batch-to-batch consistency for your critical applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C ± 5°C under an inert atmosphere (argon or nitrogen) in a dry, moisture-free environment. This compound is hygroscopic (moisture-sensitive) and easily oxidized; prolonged exposure to air, moisture, or elevated temperatures will lead to decomposition and reduced coupling efficiency.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Assay (HPLC)	≥ 97.0%
Water Content (KF)	≤ 0.5%
Solubility	Clear, colorless solution in anhydrous acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.