Description

1-{2’-o-[(2-Cyanoethoxy)(Diisopropyl Amino)Phosphino]-3’-o-[(4,4’-Dimethoxytriphenyl)Methyl]-?-L-Threofuranosyl} Thymine is a sophisticated phosphoramidite monomer, a critical building block in the automated synthesis of oligonucleotides. This compound matters because it enables the precise and efficient incorporation of thymine nucleotides into synthetic DNA and RNA strands, which is foundational for modern genetic research and therapeutic development. It is primarily needed by biotechnology and pharmaceutical companies engaged in antisense therapy, siRNA development, PCR primer synthesis, and diagnostic probe manufacturing.

Application

• Solid-Phase Oligonucleotide Synthesis: As a key phosphoramidite monomer for the automated, stepwise assembly of DNA and RNA sequences on solid supports.
• Therapeutic Oligonucleotide Production: For the synthesis of antisense oligonucleotides (ASOs), small interfering RNA (siRNA), and aptamers used in targeted drug development.
• Research & Diagnostic Probe Synthesis: Manufacturing primers for Polymerase Chain Reaction (PCR), sequencing, and fluorescently labeled probes for genomic hybridization assays.
• Synthetic Biology: Constructing custom genes, gene fragments, and other synthetic nucleic acid constructs for metabolic engineering and functional genomics.
• Next-Generation Sequencing (NGS): Production of oligonucleotide libraries and adapters essential for high-throughput sequencing platforms.
• CRISPR-Cas9 System Components: Synthesis of guide RNAs (gRNAs) and other nucleic acid components for gene editing applications.

Basic Information

Product Name	1-{2’-o-[(2-Cyanoethoxy)(Diisopropyl Amino)Phosphino]-3’-o-[(4,4’-Dimethoxytriphenyl)Methyl]-?-L-Threofuranosyl} Thymine
CAS No.	325683-94-1
Molecular Formula	C43H51N4O8P
Molecular Weight	782.87 g/mol
Synonyms	5'-O-(4,4'-Dimethoxytrityl)-2'-deoxythymidine 3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite; dT Phosphoramidite (DMT-protected); Thymidine 3'-phosphoramidite; 5'-DMT-2'-deoxythymidine-3'-(2-cyanoethyl N,N-diisopropyl)phosphoramidite; dT-CE Phosphoramidite; DNA Synthesis Phosphoramidite Monomer (dT); 2'-Deoxythymidine Phosphoramidite; T Phosphoramidite
EINECS	Contact for details

Quality Control

Our phosphoramidite monomers are manufactured under strict quality control protocols in a controlled environment to ensure the high purity and consistency required for sensitive oligonucleotide synthesis. Each batch undergoes comprehensive analysis, including HPLC purity assay, 31P NMR and 1H NMR for structural confirmation, and moisture content analysis. A detailed Certificate of Analysis (COA) is provided with every shipment, documenting all critical quality parameters.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a dry, moisture-free environment. This product is hygroscopic (moisture-sensitive) and easily oxidized; prolonged exposure to air, moisture, or elevated temperatures will lead to decomposition and reduced coupling efficiency.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms to reference standard
Identification (31P NMR)	Conforms to reference standard
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 0.5%
Solubility	Clear, colorless solution in anhydrous acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.