Description

n-Succinimidyl-5-(2-pyridyldithio)valerate is a heterobifunctional crosslinking reagent featuring an amine-reactive NHS ester and a sulfhydryl-reactive pyridyldithio group. This compound is essential for creating stable, cleavable conjugates between proteins, peptides, or other biomolecules containing primary amines and thiol groups. It is a critical tool for researchers and manufacturers in bioconjugation, antibody-drug conjugate (ADC) development, and diagnostic assay production. The product is supplied under the CAS registry number 317331-86-5.

Application

• Antibody-Drug Conjugate (ADC) Linker: A key reagent for constructing cleavable, disulfide-bridged ADCs, connecting cytotoxic payloads to monoclonal antibodies.
• Protein-Protein/Peptide Conjugation: Enables the site-specific crosslinking of different proteins or peptides for creating bispecific constructs or protein-polymer hybrids.
• Enzyme and Protein Labeling: Used to attach fluorescent dyes, biotin, or other reporter molecules to proteins via amine and thiol groups for detection and imaging.
• Surface Immobilization Chemistry: Facilitates the oriented covalent attachment of biomolecules onto sensor chips, nanoparticles, or chromatographic resins.
• Diagnostic Reagent Development: Employed in the synthesis of enzyme-antibody or hapten-carrier protein conjugates for immunoassays (ELISA, lateral flow tests).
• Polymer Functionalization: Used to introduce reactive thiol or pyridyldithio groups onto polymers or dendrimers for advanced drug delivery systems.

Basic Information

Product Name	n-Succinimidyl-5-(2-Pyridyldithio)Valerate
CAS No.	317331-86-5
Molecular Formula	C16H17N3O5S2
Molecular Weight	395.45 g/mol
Synonyms	SPDP-VAL; 5-(2-Pyridyldithio)valeric acid N-hydroxysuccinimide ester; N-Succinimidyl 5-(2-pyridyldithio)valerate; 2,5-Pyrrolidinedione, 1-[5-[(2-pyridinyldithio)-1-oxopentyl]oxy]-; SPDBV; LC-SPDP; NHS-PDPV; Valeric acid, 5-(2-pyridinyldithio)-, 2,5-dioxo-1-pyrrolidinyl ester
EINECS	Contact for details

Quality Control

Our n-Succinimidyl-5-(2-pyridyldithio)valerate is manufactured under strict quality systems to ensure batch-to-batch consistency for sensitive bioconjugation applications. Each lot is characterized by advanced analytical techniques including HPLC, NMR, and mass spectrometry to confirm identity, purity, and functional integrity. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake. For long-term storage, keep under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Assay	≥ 90.0% (by functional titration)
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.