Description

Citric Acid-1,5-13C2 is a stable isotope-labeled analog of citric acid, specifically enriched with carbon-13 at the 1 and 5 positions. This high-purity compound is essential for advanced analytical and metabolic research, providing a critical tracer for precise quantification and pathway elucidation. It is primarily required by pharmaceutical R&D laboratories, academic research institutions, and diagnostic manufacturers for applications in mass spectrometry, NMR spectroscopy, and metabolic flux analysis.

Application

• Internal Standard for Quantitative Analysis: Used as a certified reference material in LC-MS and GC-MS for the accurate quantification of endogenous citric acid and related metabolites in biological samples.
• Metabolic Flux Analysis (MFA): Serves as a crucial tracer to study the citric acid cycle (Krebs cycle) dynamics, enzyme activities, and metabolic pathways in cells, tissues, or whole organisms.
• NMR Spectroscopy Studies: Provides distinct spectral signatures for probing molecular structure, reaction mechanisms, and metabolic processes without natural abundance background interference.
• Pharmaceutical Drug Metabolism & Pharmacokinetics (DMPK): Employed in radiolabeled and stable isotope studies to track the absorption, distribution, metabolism, and excretion of new drug candidates.
• Clinical Diagnostics Development: Used in the calibration and validation of assays for inborn errors of metabolism, such as those detecting cycle intermediates.
• Biochemical Research: Applied in enzymatic studies to investigate kinetics and inhibition of aconitase, citrate synthase, and other enzymes of the TCA cycle.

Basic Information

Item	Detail
Product Name	Citric Acid-1,5-13C2
CAS No.	302912-06-7
Molecular Formula	C613C2H8O7
Molecular Weight	~194.10 g/mol
Synonyms	1,5-13C2-Citric Acid; [1,5-13C2]Citric Acid; Citric Acid (1,5-13C2); 2-Hydroxy-1,2,3-propanetricarboxylic-1,5-13C2 Acid; 3-Carboxy-3-hydroxypentanedioic-1,5-13C2 Acid; (1,5-13C2)Citrate; Isotopic Citric Acid; 13C2-Labeled Citric Acid
EINECS	Contact for details

Quality Control

Every batch of Citric Acid-1,5-13C2 is manufactured and analyzed under strict quality systems to ensure exceptional isotopic and chemical purity. Our products undergo rigorous quality testing, including isotopic enrichment analysis by NMR and MS, chemical purity by HPLC, and identity confirmation. Certificates of Analysis (COA) detailing batch-specific results for isotopic purity, chemical purity, and residual solvents are provided and traceable to relevant standards.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent absorption of atmospheric moisture, which can affect stability and accurate weighing.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Isotopic Purity (13C)	≥ 99 atom % 13C (at positions 1 & 5)
Chemical Purity (HPLC)	≥ 98.0%
Isotopic Enrichment (MS)	≥ 99% M+2
Water Content (KF)	≤ 0.5% w/w
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.