Description

1,4-Dithiothreitol-D10 is a deuterated isotopologue of the classic reducing agent 1,4-Dithiothreitol (DTT), specifically designed for research applications requiring stable isotope labeling. This compound is essential for providing a non-reactive internal standard in mass spectrometry, enabling precise quantification and tracking in complex biochemical studies. It is primarily needed by analytical chemists and researchers in pharmaceutical development, proteomics, and metabolic pathway analysis where high-fidelity measurement of thiol-containing compounds is critical.

Application

• Internal Standard for LC-MS/MS: Used as a deuterated internal standard for the accurate quantification of DTT and related thiols in complex biological matrices via mass spectrometry.
• Proteomics Research: Employed in the study of protein folding, disulfide bond reduction, and protein structure analysis under conditions that benefit from isotopic labeling.
• Metabolic Tracing: Facilitates the tracking of thiol-disulfide exchange pathways and redox metabolism in cellular systems using stable isotope techniques.
• Pharmaceutical Analysis: Supports method development and validation in pharmacokinetic and bioanalytical studies for drugs with thiol moieties.
• Biochemical Reagent Manufacturing: Serves as a high-purity labeled raw material in the production of specialized diagnostic kits and research reagents.
• Quality Control Reference: Acts as a certified reference material (CRM) in analytical laboratories to ensure the accuracy and precision of assay methods.

Basic Information

Product Name	1,4-Dithiothreitol-D10
CAS No.	302912-05-6
Molecular Formula	C4H2D10O2S2
Molecular Weight	162.33 g/mol (deuterated form)
Synonyms	DTT-D10; (2S,3S)-1,4-Dimercapto-2,3-butanediol-d10; Deuterated DTT; Cleland's Reagent-d10; Dithiothreitol-d10; 1,4-Dithio-DL-threitol-d10; meso-1,4-Dimercapto-2,3-butanediol-d10; Isotopically Labeled DTT
EINECS	Contact for details

Quality Control

Our 1,4-Dithiothreitol-D10 is manufactured under strict quality systems to ensure high isotopic purity and chemical integrity. Each batch is supported by a comprehensive Certificate of Analysis (COA) detailing parameters such as isotopic enrichment (Atom % D), chemical purity, and absence of critical impurities. We adhere to relevant standards for research chemicals, and custom synthesis with specific purity or packaging requirements is available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C under an inert atmosphere (e.g., nitrogen or argon) to minimize oxidation and moisture absorption. The product is hygroscopic and light-sensitive; allow the sealed vial to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Purity (Atom % D)	≥ 98.0%
Water Content (KF)	≤ 0.5%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.