Description

3-Chloro-3-(4-Chlorophenyl)-3H-Diazirine is a specialized diazirine-based photoaffinity labeling reagent, a critical tool for probing biomolecular interactions. Its core value lies in generating highly reactive carbene intermediates upon photolysis, enabling the covalent cross-linking of proteins, nucleic acids, and other biological molecules for structural and functional studies. This compound is essential for researchers and developers in pharmaceutical discovery, chemical biology, and proteomics who require precise, irreversible labeling to map binding sites and understand complex biological mechanisms.

Application

• Photoaffinity Labeling (PAL): A key reagent for covalent cross-linking in the study of protein-ligand, protein-protein, and protein-nucleic acid interactions.
• Chemical Biology & Proteomics: Used to identify and characterize unknown biological targets of small molecule drugs and natural products.
• Drug Discovery: Facilitates the mapping of drug binding sites and the elucidation of mechanisms of action during early-stage pharmaceutical R&D.
• Bioconjugation & Material Science: Serves as a photochemical handle for the site-specific modification of polymers, surfaces, and biomaterials.
• Structural Biology: Aids in the stabilization of transient complexes for analysis via techniques like mass spectrometry and X-ray crystallography.
• Organic Synthesis: Acts as a versatile precursor for the synthesis of more complex, functionalized diazirine derivatives and other chlorinated intermediates.

Basic Information

Product Name	3-Chloro-3-(4-Chlorophenyl)-3H-Diazirine
CAS No.	39184-66-2
Molecular Formula	C7H4Cl2N2
Molecular Weight	187.03 g/mol
Synonyms	3-Chloro-3-(4-chlorophenyl)diazirine; 4-Chlorophenyl(chloro)diazirine; (4-Chlorophenyl)chlorodiazirine; 3-Chloro-3-(p-chlorophenyl)diazirine; 3-Chloro-3-(4'-chlorophenyl)diazirine; Diaziridine, 3-chloro-3-(4-chlorophenyl)-; 3H-Diazirine, 3-chloro-3-(4-chlorophenyl)-
EINECS	Contact for details

Quality Control

Our 3-Chloro-3-(4-Chlorophenyl)-3H-Diazirine is manufactured under strict quality protocols to ensure high purity and batch-to-batch consistency, critical for sensitive photochemical applications. Each lot is supported by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and stability parameters. We adhere to cGMP principles where applicable, and our quality system is designed to meet the stringent requirements of research and development laboratories.

Storage

Preserve in a tightly closed container, protected from light. Store in a freezer at -20°C or below under an inert atmosphere (e.g., argon or nitrogen) to maximize stability. The container should be kept in a dry, well-ventilated area away from heat and incompatible materials. Allow the sealed container to equilibrate to room temperature before opening to prevent moisture condensation.

Specification

Item	Specification
Appearance	Yellow to orange crystalline solid or solution
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Solvent (if supplied as solution)	Specified (e.g., Toluene, Hexane)
Water Content (KF)	< 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.