Description

6-Diazo-5-Oxo-L-Norleucine is a diazoketone derivative of the amino acid norleucine, known for its role as a potent and irreversible inhibitor of the enzyme glutamine amidotransferase. This compound matters significantly in biochemical research for its ability to selectively block glutamine-dependent biosynthetic pathways, making it a critical tool for studying cellular metabolism and enzyme function. It is primarily needed by researchers in pharmaceutical development, cancer biology, and enzymology for investigating novel therapeutic targets and metabolic regulation.

Application

• Biochemical Research: Used as a specific inhibitor to study glutamine metabolism and the function of glutamine-utilizing enzymes in cell culture and in vitro assays.
• Anticancer Agent Screening: Serves as a reference compound in drug discovery programs targeting glutamine addiction in various cancer cell lines.
• Enzyme Mechanism Studies: Employed to elucidate the catalytic mechanisms of glutamine amidotransferases and related enzymes.
• Metabolic Pathway Analysis: A key tool for probing nitrogen metabolism and nucleotide biosynthesis pathways in metabolic research.
• Pharmaceutical Intermediates: Can act as a specialized building block or precursor in the synthesis of more complex bioactive molecules.

Basic Information

Product Name	6-Diazo-5-Oxo-L-Norleucine
CAS No.	157-03-9
Molecular Formula	C6H9N3O3
Molecular Weight	171.16 g/mol
Synonyms	DON; 6-Diazo-5-oxonorleucine; L-6-Diazo-5-oxonorleucine; Diazooxonorleucine; NSC 7365; Antibiotic DON; (2S)-2-Amino-6-diazo-5-oxohexanoic acid
EINECS	205-851-5

Quality Control

Our 6-Diazo-5-Oxo-L-Norleucine is produced and handled under strict quality management protocols. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for research applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and related substances is provided with every shipment to guarantee traceability and consistency for your critical work.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability. For long-term storage, consider keeping the container under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Optical Rotation	Contact for details
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.