Description

p-Nitrobenzyl 1-Thio-D-Galactopryranoside is a specialized thioglycoside derivative used as a critical reagent in biochemical and enzymatic research. This compound serves as a chromogenic substrate for the detection and assay of β-galactosidase activity, a key enzyme in molecular biology and diagnostic applications. It is essential for researchers and manufacturers in the biotechnology, pharmaceutical, and diagnostic kit production sectors who require high-purity, reliable substrates for sensitive enzymatic studies.

Application

• Enzymatic Substrate for β-Galactosidase: Primary use as a chromogenic substrate in assays to detect and quantify β-galactosidase enzyme activity.
• Molecular Biology Research: Used in cloning, gene expression studies, and reporter gene assays (e.g., lacZ system) in microbiology and genetics.
• Diagnostic Kit Manufacturing: A key component in the formulation of diagnostic kits for clinical and research laboratories to identify bacterial species (like E. coli) or genetic markers.
• Biochemical Reagent: Employed in enzyme kinetics studies and inhibitor screening within drug discovery and biochemical pathway analysis.
• Quality Control in Biotechnology: Used as a standard or control reagent in the quality assurance processes for enzyme production and purification.

Basic Information

Product Name	p-Nitrobenzyl 1-Thio-D-Galactopryranoside
CAS No.	35785-19-4
Molecular Formula	C₁₃H₁₇NO₇S
Molecular Weight	331.34 g/mol
Synonyms	PNP-β-D-Galactopyranoside; p-Nitrophenyl β-D-Galactopyranoside; 4-Nitrophenyl β-D-galactopyranoside; p-Nitrophenyl β-D-galactoside; 4-Nitrophenyl β-D-galactoside; β-Galactosidase Substrate; PNPG; ONPG (o-Nitrophenyl-β-D-galactopyranoside is a related common substrate, often listed as a synonym in searches).
EINECS	Contact for details

Quality Control

Our p-Nitrobenzyl 1-Thio-D-Galactopryranoside is manufactured under strict quality systems to ensure batch-to-batch consistency and high purity suitable for sensitive research applications. We provide comprehensive Certificates of Analysis (COA) with each batch, detailing purity, identity, and impurity profiles. While not typically a USP/EP monograph item, production can adhere to cGMP principles for research-grade materials upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation from atmospheric moisture.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Melting Point	Contact for details
Solubility	Soluble in water, DMSO, DMF
Heavy Metals	< 10 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.