Description

Dl-4-Hydroxyphenylalanine-3,3-D2 is a deuterium-labeled isotopologue of the amino acid derivative DL-tyrosine, specifically deuterated at the 3,3 positions of the phenyl ring. This compound is a critical tool in pharmaceutical research and development, enabling advanced pharmacokinetic and metabolic studies through mass spectrometry and NMR spectroscopy. It is primarily utilized by research institutions, pharmaceutical companies, and CROs (Contract Research Organizations) engaged in drug discovery, biomarker analysis, and mechanistic biochemical investigations.

Application

• Internal Standard for Mass Spectrometry: Serves as a stable, non-radioactive isotopic internal standard for the precise quantification of tyrosine and related metabolites in complex biological matrices via LC-MS/MS.
• Metabolic Pathway Tracing: Used in tracer studies to elucidate the metabolic fate, turnover rates, and biosynthetic pathways of aromatic amino acids in vivo and in vitro.
• Drug Metabolism and Pharmacokinetics (DMPK): Essential for studying the absorption, distribution, metabolism, and excretion (ADME) of tyrosine-based drug candidates.
• Biochemical Research: Aids in mechanistic studies of enzymes like tyrosine hydroxylase and tyrosine aminotransferase, where deuterium labeling can help elucidate kinetic isotope effects.
• Proteomics and Biomarker Discovery: Facilitates relative and absolute quantification in quantitative proteomics and the discovery of disease-related biomarkers.
• NMR Spectroscopy: Provides a useful probe for nuclear magnetic resonance studies due to the distinct signal of deuterium, aiding in structural and dynamic analysis.

Basic Information

Product Name	Dl-4-Hydroxyphenylalanine-3,3-D2
CAS No.	35693-18-6
Molecular Formula	C9H9D2NO3
Molecular Weight	183.20 g/mol (for monoisotopic mass)
Synonyms	DL-Tyrosine-3,3-d2; (RS)-2-Amino-3-(4-hydroxyphenyl-3,3-d2)propanoic acid; 3-(4-Hydroxyphenyl-3,3-d2)alanine; DL-4-Hydroxyphenyl-d2-alanine; DL-β-(4-Hydroxyphenyl-3,3-d2)alanine; DL-3-(4-Hydroxyphenyl-3,3-d2)alanine; Tyrosine-d2 (DL-form); H-d2-Phe(4-OH)-OH
EINECS	Contact for details

Quality Control

Our Dl-4-Hydroxyphenylalanine-3,3-D2 is manufactured under strict quality control protocols to ensure high chemical purity and isotopic enrichment suitable for sensitive analytical applications. Each batch is characterized by advanced spectroscopic and chromatographic techniques, including NMR, HPLC, and MS, to confirm identity, chemical purity, and deuterium incorporation. Certificates of Analysis (COA) detailing lot-specific results are provided and can be tailored to meet specific research or regulatory requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This material is hygroscopic (moisture-sensitive); therefore, containers must be kept tightly sealed in a dry environment to prevent absorption of moisture. For long-term storage, consider storing under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Deuterium Incorporation (NMR/MS)	≥ 98 atom % D
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.