Description

3-Chloro-3-(Methyl-D 3 )-3H-Diazirine is a deuterated, highly reactive photoaffinity labeling reagent used for covalent cross-linking in molecular structure elucidation. Its core value lies in enabling precise, irreversible binding to target molecules upon photolysis, which is critical for studying protein-ligand interactions and biological pathways. This compound is essential for researchers and developers in pharmaceutical R&D, chemical biology, and proteomics who require advanced tools for mapping binding sites and understanding molecular mechanisms.

Application

• Photoaffinity Labeling (PAL): Primary application for irreversible covalent tagging of biological targets (e.g., proteins, enzymes, receptors) upon UV light activation.
• Chemical Biology & Proteomics Research: Used as a molecular probe to identify and characterize drug binding sites, protein-protein interactions, and active sites.
• Deuterium-Labeled Cross-linker: The deuterated methyl group provides a mass tag for detection and analysis via mass spectrometry (MS), aiding in the study of complex biological systems.
• Pharmaceutical Development: Employed in early-stage drug discovery for target identification and validation, helping to elucidate the mechanism of action of novel compounds.
• Material Science: Potential use in surface modification and creating covalently bonded functional layers on substrates.
• Radical Chemistry Studies: Serves as a precursor for generating carbene intermediates under mild photolytic conditions for synthetic applications.

Basic Information

Product Name	3-Chloro-3-(Methyl-D 3 )-3H-Diazirine
CAS No.	35496-73-2
Molecular Formula	C2D3ClN2
Molecular Weight	105.52 g/mol
Synonyms	3-Chloro-3-(trideuteriomethyl)diazirine; 3-Chloro-3-(trideuteromethyl)-3H-diazirine; 3-Chloro-3-(methyl-d3)diazirine; Diazirine, 3-chloro-3-(methyl-d3)-; Deuterated 3-Chloro-3-methyldiazirine; (Trideuteriomethyl)chlorodiazirine; Photoaffinity Labeling Reagent (Deuterated); CD3-Labeled Chlorodiazirine
EINECS	Contact for details

Quality Control

Our 3-Chloro-3-(Methyl-D 3 )-3H-Diazirine is produced and handled under strict quality protocols to ensure high purity and batch-to-batch consistency, suitable for sensitive research applications. Each batch is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, isotopic enrichment, and stability data. We adhere to cGMP principles for manufacturing and quality assurance to support our clients in regulated research environments.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C (refrigerated) or as specified on the label or COA. Due to its highly volatile and strictly light-sensitive nature, this product must be handled under inert atmosphere (e.g., nitrogen or argon) when opened and used promptly to maintain stability. Keep away from heat, sparks, and open flame.

Specification

Item	Specification
Appearance	Colorless to pale yellow liquid
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Deuterium Enrichment (NMR/MS)	≥ 98 atom % D
Water Content (KF)	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.