Description

3-Indoxyl Phosphate, p-Toluidine Salt is a chromogenic substrate widely used in enzymatic assays and diagnostic applications. This compound is critical for the detection of alkaline phosphatase activity, producing a visible indigo blue color upon enzymatic hydrolysis. It is an essential reagent for researchers and manufacturers in the life sciences, clinical diagnostics, and biotechnology sectors, where precise and sensitive detection methods are required.

Application

• Enzymatic Substrate for Alkaline Phosphatase (ALP): Primary chromogenic agent in biochemical assays and diagnostic kits for detecting ALP activity.
• Immunohistochemistry (IHC) & In Situ Hybridization (ISH): Used as a staining substrate for visualizing target antigens or nucleic acids in tissue sections.
• Western, Northern, and Southern Blotting: Employed in non-radioactive detection systems for proteins and nucleic acids.
• ELISA (Enzyme-Linked Immunosorbent Assay): Functions as a key component in colorimetric detection systems.
• Microbiology & Cell Biology Research: Utilized in reporter gene assays and studies of gene expression and signal transduction pathways.
• Diagnostic Manufacturing: A vital raw material in the production of clinical chemistry reagents and point-of-care test devices.
• Quality Control in Pharmaceutical Production: Applied in assays to monitor enzymatic processes or contamination.

Basic Information

Product Name	3-Indoxyl Phosphate, p-Toluidine Salt
CAS No.	31699-61-3
Molecular Formula	C15H17N2O4P
Molecular Weight	320.28 g/mol
Synonyms	5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt; BCIP p-toluidine salt; 3-Indolyl phosphate p-toluidine salt; Indoxyl phosphate toluidine salt; 5-Bromo-4-chloro-3-indoxyl phosphate p-toluidinium salt; BCIP; X-Phosphate
EINECS	Contact for details

Quality Control

Our 3-Indoxyl Phosphate, p-Toluidine Salt is manufactured under strict quality systems to ensure batch-to-batch consistency and performance in sensitive applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity, identity confirmation by IR and NMR spectroscopy, and residual solvent analysis. A Certificate of Analysis (COA) detailing all specifications is provided with every shipment to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. The product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation. For long-term stability, store desiccated.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMF, DMSO; slightly soluble in water
Heavy Metals	≤ 20 ppm
Residual Solvents (GC)	Meets ICH guidelines
Performance (Enzymatic Test)	Conforms to reference standard

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.